Protein Info for Psyr_2146 in Pseudomonas syringae pv. syringae B728a

Annotation: methionine aminopeptidase, type I

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00500: methionine aminopeptidase, type I
  • amino acids 5 to 251 (247 residues), 307.7 bits, E=3e-96
• PF00557: Peptidase_M24
  • amino acids 15 to 243 (229 residues), 183.3 bits, E=2.7e-58

Best Hits

Swiss-Prot: 58% identical to MAP1_ECOLI: Methionine aminopeptidase (map) from Escherichia coli (strain K12)

KEGG orthology group: K01265, methionyl aminopeptidase [EC: 3.4.11.18] (inferred from 100% identity to psb:Psyr_2146)

MetaCyc: 58% identical to methionine aminopeptidase (Escherichia coli K-12 substr. MG1655)
Methionyl aminopeptidase. [EC: 3.4.11.18]

Predicted SEED Role

"Methionine aminopeptidase (EC 3.4.11.18)" (EC 3.4.11.18)

MetaCyc Pathways

• Arg/N-end rule pathway (eukaryotic) (8/14 steps found)
• Ac/N-end rule pathway (6/21 steps found)

Isozymes

Compare fitness of predicted isozymes for: 3.4.11.18

Use Curated BLAST to search for 3.4.11.18

Sequence Analysis Tools

See Q4ZUI3 at UniProt or InterPro

Protein Sequence (263 amino acids)

>Psyr_2146 methionine aminopeptidase, type I (Pseudomonas syringae pv. syringae B728a)
MSSAIGIKTEQDLVQLRIAGRLAADVLAMITPYVKAGVSTEALDDICNEYIVKELKVIPA
NVGYHGFTKTTCISPNAVVCHGIPSATDILKEGDIVNIDVAVIKDGWYGDTSRMYLVGEV
SPLARRLVETTYEATRAGIHAVRPGATLGDIGYAIQSVAHREGFSVVREYCGHGIGRKYH
EEPQVLHYGAQGKGLRLKPGMVFTIEPMINAGRAGTRTLPDGWTVLTSDLSLSAQWEHMV
AVTSTGFELLTPWPDGTGDYPAI