Protein Info for Psyr_2138 in Pseudomonas syringae pv. syringae B728a

Annotation: Glycosyl transferase, family 2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 308 transmembrane" amino acids 263 to 278 (16 residues), see Phobius details PF13641: Glyco_tranf_2_3" amino acids 5 to 223 (219 residues), 54.4 bits, see alignment E=2.5e-18 PF00535: Glycos_transf_2" amino acids 9 to 173 (165 residues), 54.1 bits, see alignment E=2.9e-18 PF13506: Glyco_transf_21" amino acids 96 to 223 (128 residues), 23 bits, see alignment E=7.8e-09

Best Hits

Swiss-Prot: 51% identical to RFBN_SALTY: O antigen biosynthesis rhamnosyltransferase RfbN (rfbN) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K12992, rhamnosyltransferase [EC: 2.4.1.-] (inferred from 100% identity to psb:Psyr_2138)

MetaCyc: 51% identical to dTDP-Rha:alpha-D-Gal-diphosphoundecaprenol alpha-1,3-rhamnosyltransferase (Salmonella enterica enterica serovar Typhimurium str. LT2)
RXN-21844 [EC: 2.4.1.377]

Predicted SEED Role

"O antigen biosynthesis rhamnosyltransferase rfbN (EC 2.4.1.-)" (EC 2.4.1.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.-

Use Curated BLAST to search for 2.4.1.- or 2.4.1.377

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZUJ1 at UniProt or InterPro

Protein Sequence (308 amino acids)

>Psyr_2138 Glycosyl transferase, family 2 (Pseudomonas syringae pv. syringae B728a)
MAPLRVACVIPTYNGRKDLERLLDSLAAQTASFDTLIVDSSSSDGTLELAQARCANVLRI
DSKDFNHGGTRQMMVDLHPDYDVYVFMTQDAYVEDINAIANILLPFADPKVGAVCGRQLP
HKDANLLAQHARLFNYPPTSQIKTLADAGTLGIKTPFMSNSFAAYRGEALRAIGGFPRHV
ILSEDMYVTAKMLIDGWKAAYEGSAVCRHSHNYSLREEFRRYFDIGVFQAREAWIYETFG
GIGGEGMRYVKSELKFLGPRRILWWPVSFVRNALKLLAYKLSRQEKRLPRGVKKKLGMYA
RYWDSPYA