Protein Info for Psyr_2124 in Pseudomonas syringae pv. syringae B728a

Annotation: Benzoate transport

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 449 transmembrane" amino acids 31 to 53 (23 residues), see Phobius details amino acids 66 to 85 (20 residues), see Phobius details amino acids 96 to 115 (20 residues), see Phobius details amino acids 122 to 143 (22 residues), see Phobius details amino acids 154 to 175 (22 residues), see Phobius details amino acids 185 to 205 (21 residues), see Phobius details amino acids 260 to 283 (24 residues), see Phobius details amino acids 302 to 323 (22 residues), see Phobius details amino acids 330 to 347 (18 residues), see Phobius details amino acids 353 to 371 (19 residues), see Phobius details amino acids 391 to 416 (26 residues), see Phobius details amino acids 422 to 443 (22 residues), see Phobius details TIGR00895: MFS transporter, aromatic acid:H+ symporter (AAHS) family" amino acids 14 to 413 (400 residues), 495 bits, see alignment E=7.8e-153 PF07690: MFS_1" amino acids 36 to 290 (255 residues), 114.6 bits, see alignment E=7.4e-37 amino acids 269 to 438 (170 residues), 50.3 bits, see alignment E=2.7e-17 PF00083: Sugar_tr" amino acids 40 to 229 (190 residues), 91.5 bits, see alignment E=8.9e-30 PF06779: MFS_4" amino acids 52 to 208 (157 residues), 32 bits, see alignment E=1.4e-11

Best Hits

Swiss-Prot: 86% identical to PCAK_PSEAE: 4-hydroxybenzoate transporter PcaK (pcaK) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K08195, MFS transporter, AAHS family, 4-hydroxybenzoate transporter (inferred from 100% identity to psb:Psyr_2124)

Predicted SEED Role

"4-hydroxybenzoate transporter" in subsystem Cinnamic Acid Degradation or Gentisare degradation or Phenylpropanoid compound degradation or Salicylate and gentisate catabolism or p-Hydroxybenzoate degradation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZUK5 at UniProt or InterPro

Protein Sequence (449 amino acids)

>Psyr_2124 Benzoate transport (Pseudomonas syringae pv. syringae B728a)
MTLSAGSPAPSTLDVQAFINAQPLSGYQWRVVALCFLIVFLDGLDTAAMGFIAPALSQDW
GIDRASLGPVMSAALIGMVFGALGSGPLADRFGRKVVLVVAVLLFGLFSLASAYSSNIDQ
LLVLRLLTGLGLGAAMPNATTLLSEYTPERLKSLLVTSMFCGFNLGMACGGFVSAKLIPS
MGWHSLLMLGGILPLLLGLVLMVWLPESARFLVVRNRGAERIRKVLAPIAPKALAGITDF
SVPEQKTVSSRNVLKVIFSGTYSAGTLLLWLTYFMGLVIVYLLTSWLPTLMRDSGASMEQ
SAFIGALFQFGGVLSAVGVGWAMDRYNPHKVIGCAYALAGVFAWLVGQSLGNVALLATLV
LLAGMCINGAQSAMPSLAARFYPTQGRATGVSWMLGIGRFGAILGAWAGATLLGLGWTFE
QVLTALVIPAALAAIAVLIKGWVSHSDAT