Protein Info for Psyr_2113 in Pseudomonas syringae pv. syringae B728a

Annotation: multi-sensor hybrid histidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1206 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details transmembrane" amino acids 538 to 559 (22 residues), see Phobius details PF00497: SBP_bac_3" amino acids 66 to 276 (211 residues), 93.3 bits, see alignment E=5.8e-30 amino acids 305 to 522 (218 residues), 97.1 bits, see alignment E=4.1e-31 PF00989: PAS" amino acids 579 to 691 (113 residues), 29.4 bits, see alignment E=2.4e-10 PF08448: PAS_4" amino acids 586 to 696 (111 residues), 41.9 bits, see alignment E=3.6e-14 PF00512: HisKA" amino acids 714 to 780 (67 residues), 64.4 bits, see alignment 2.8e-21 PF02518: HATPase_c" amino acids 828 to 941 (114 residues), 92.7 bits, see alignment E=7.2e-30 PF00072: Response_reg" amino acids 967 to 1081 (115 residues), 83 bits, see alignment E=6e-27 PF01627: Hpt" amino acids 1113 to 1178 (66 residues), 36.2 bits, see alignment 2.1e-12

Best Hits

KEGG orthology group: K07679, two-component system, NarL family, sensor histidine kinase EvgS [EC: 2.7.13.3] (inferred from 100% identity to psb:Psyr_2113)

Predicted SEED Role

"Hybrid sensory histidine kinase in two-component regulatory system with EvgA" in subsystem Orphan regulatory proteins

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZUL6 at UniProt or InterPro

Protein Sequence (1206 amino acids)

>Psyr_2113 multi-sensor hybrid histidine kinase (Pseudomonas syringae pv. syringae B728a)
MSKRFRQLLILATSLCIGWGAEAGVHAADHYTLLGRSSPAHMDVKLDNNQWQWVRGQREL
ILGTSNPDYPPFDITMSGNDYEGITADYAGIISDALDLPIRVQRFETRDAAIKALGAGQI
DLLGTANGFEAVDRTIRLSQPYSVDQPVLVTRVGDNRPLNEGLKGMRLSMVYHYLPPAEV
EASYPGATLKTYPSFQNAINAVAFNQADVFLGDTISTQYIINKGYLNNVQMSNFGKHEPN
GFSFAVSSENPQLLGIINQTLKAIPVDERVNISRRWSTGSDLMLTDQKLQLTQREERWIA
QHPTVRVVVNEAYAPLTFFDAKGNFRGITADLLELVRLRTGLRFDVKRSPSLTDMLNQVS
EGQADMIGALVSSDAREDRLSFTRPYIDNSFVLVTRKEPGAPSYLEQMDGKRLAITQGSP
VLDWLSRKYPRVVPLEIDNPFQALDMLSLGRAEGAVISLLSADYFLSSGIFEERLQMTAT
VGEDPALISMATSRNAIELSSILDKALASIAPQDLAAINNRWRVYTPSRANWHDYERLIY
QIVVGAGLLLLGLLAWNAWMRRQIRQREAAERALGDQFEFMRALVEGTPHPIYVRDREGM
LRMCNDSYLRVFSAQREDIIGKSATEGVLGNAFEAREYAADYQRVMASNTALILDRPLRI
GDRQLTIYHWILPFRDSLGEVQGIIGGWIDISERRQLIEDLQAAKEQADAASRAKSTFLA
TMSHEIRTPMNAVIGMLELALKRADHGELDRSAIEVAYSSANDLLDLIGDILDIARIESG
RLSLNPERANLRRLVEAVLRVFDGLARQKDLELVLELDSRINADVLIDPLRFKQILSNLV
SNAIKFTPMGRIKVVLLAAESPDPQLLHLHITVQDSGIGISVEDQQRLFEPFAQADNTGQ
MARTGAGLGLVICRSLCMMMGGNLSLDSTPGHGTRISMNLVLTTLEPLTGQPVVAQPAAV
VHQTLRILIVDDHPANRLLLCQQLRFLGHHCEMAENGAQGLDRWKNDAFDLVVADCNMPI
MNGYDMTAAIREQERACERQRCPVWGFTANAQPDEIERCRAAGMDDCLFKPISLSMLSER
LTAIAPLAPARALPFSLDSVSNLTGDRPEMVERLLAQLLHSNHEDRLLLARLMPENNRQK
TRDLAHRIKGAARIITASRVVDACDALELACEPDTPDEQFYTCQQTVELAMIELEDALML
QQAKPD