Protein Info for Psyr_2099 in Pseudomonas syringae pv. syringae B728a

Annotation: assimilatory nitrate reductase (NADH) alpha subunit apoprotein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 906 PF04879: Molybdop_Fe4S4" amino acids 11 to 58 (48 residues), 60 bits, see alignment 3.5e-20 PF00384: Molybdopterin" amino acids 69 to 494 (426 residues), 249.5 bits, see alignment E=1.1e-77 PF01568: Molydop_binding" amino acids 593 to 702 (110 residues), 87.7 bits, see alignment E=1.1e-28 PF04324: Fer2_BFD" amino acids 854 to 900 (47 residues), 35.9 bits, see alignment 1.5e-12

Best Hits

KEGG orthology group: K02567, periplasmic nitrate reductase NapA [EC: 1.7.99.4] (inferred from 100% identity to psb:Psyr_2099)

Predicted SEED Role

"Assimilatory nitrate reductase large subunit (EC:1.7.99.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.99.4)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.7.99.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZUN0 at UniProt or InterPro

Protein Sequence (906 amino acids)

>Psyr_2099 assimilatory nitrate reductase (NADH) alpha subunit apoprotein (Pseudomonas syringae pv. syringae B728a)
MNSLALHTPRITASTCCYCGVGCGVLIEHDGEHILGVSGDPKHPANFGKLCSKGSSLHLT
GDIEARALYPELRLSKGLARSVTHWDTALEHAANVFAQTIREHGPDSVAFYISGQLLTED
YYAFNKLARALIGTNNIDSNSRLCMSSAVAGYKRSLGADAPPCSYEDIELSDCVMIVGSN
MAYAHPILFRRLEEAKNHRPQMKLIVIDPRRTDTCELADLHLAIQPGTDVALFHGILHIL
MSERLIDPAFIAAHTQGYEELATLVHDYPPEHVAALCGITLEQLHTCARWVGEAPAFLSL
WCMGLNQSTAGSAKNSALINLHLATGQIGKPGAGPFSLTGQPNAMGGRETGSLSNLLPGH
RDAANPQHRQEVADYWGVDSLPASPGLSAIELFEQMQNGTIKALWIACTNPAQSLPDQNR
IRQALETCPFVVLQEAFKTTETARYADLLLPAASWGEKEGTVTNSERRISNVRKAIQAPG
EARSDWAITVDFAQRLQQRLQPDAPALFDFATPKALFDEFKGLTVGRDLDMSGIDRELID
QQGPQQWPFPRGASSGTQRLYSEGIFPTDSGRAQFLSEPYIAARELRDADYPLTLNTGRL
RDQWHGMSRTGTAARLFGHVSEAVLSINPQDMLSHDLQPGDLVKLISRRGELLLPVSSED
SVAVGQAFVPMHWGDRFLKGGVNVLTQPAFDPISKQPELKHAGVRIEKAHLPWQFFALVE
GNVQQRMETLRALCDTFTYLSMGLAGRGRPALVIRAASAEAPDSALLQQIDRVLGLDEGP
VMAYDDPKRSIGKRVRIDDDRITAIRLAGETLARHWLQTLWLEERVEASLRRWLLAPLSS
EPGKDSTQTRDKTLCNCMNVSQNAVMSAIERGLDLNQLKTQLGCGTQCGSCVPEIRRLIH
TVAVTE