Protein Info for Psyr_2085 in Pseudomonas syringae pv. syringae B728a

Annotation: methylisocitrate lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 297 TIGR02317: methylisocitrate lyase" amino acids 9 to 293 (285 residues), 438.1 bits, see alignment E=6.5e-136 PF13714: PEP_mutase" amino acids 17 to 254 (238 residues), 153.2 bits, see alignment E=9.2e-49 PF00463: ICL" amino acids 83 to 179 (97 residues), 46.3 bits, see alignment E=2.3e-16

Best Hits

Swiss-Prot: 78% identical to PRPB_CUPNE: 2-methylisocitrate lyase (prpB) from Cupriavidus necator

KEGG orthology group: K03417, methylisocitrate lyase [EC: 4.1.3.30] (inferred from 100% identity to psb:Psyr_2085)

MetaCyc: 79% identical to 2-methylcitrate lyase (Cupriavidus necator)
Methylisocitrate lyase. [EC: 4.1.3.30]

Predicted SEED Role

"Methylisocitrate lyase (EC 4.1.3.30)" in subsystem Methylcitrate cycle or Propionate-CoA to Succinate Module (EC 4.1.3.30)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.3.30

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZUP4 at UniProt or InterPro

Protein Sequence (297 amino acids)

>Psyr_2085 methylisocitrate lyase (Pseudomonas syringae pv. syringae B728a)
MSHDNSTPGQRFRDAVTSEQPLQVVGAINANHALLARRAGFKAIYLSGGGVAAGSLGIPD
LGITGLEDVLIDVRRITDVCDLPLLVDVDTGFGSSAFNVARTVRSMIKAGAAAIHIEDQV
GAKRCGHRPNKEIVSQQEMVDRIKAAVDARTDDSFVIMARTDALAVEGLESALERAAACI
EAGADMVFPEAITELAMYKQFANRAGVPILANITEFGATPLFTVDELREADVSLVLYPLS
AFRAMNKAAENVYGAIRRDGSQKNVIDSMQTRMELYDAIDYHTFEQKLDALFAQKKG