Protein Info for Psyr_2017 in Pseudomonas syringae pv. syringae B728a

Annotation: Heat shock protein Hsp90:ATP-binding region, ATPase-like protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 637 PF13589: HATPase_c_3" amino acids 34 to 160 (127 residues), 39.1 bits, see alignment E=1.1e-13 PF02518: HATPase_c" amino acids 34 to 186 (153 residues), 28.8 bits, see alignment E=2.1e-10 PF00183: HSP90" amino acids 229 to 632 (404 residues), 432.8 bits, see alignment E=3.5e-133

Best Hits

Swiss-Prot: 100% identical to HTPG_PSEU2: Chaperone protein HtpG (htpG) from Pseudomonas syringae pv. syringae (strain B728a)

KEGG orthology group: K04079, molecular chaperone HtpG (inferred from 100% identity to psb:Psyr_2017)

MetaCyc: 60% identical to chaperone protein HtpG (Escherichia coli K-12 substr. MG1655)
Non-chaperonin molecular chaperone ATPase. [EC: 3.6.4.10, 5.6.1.7]

Predicted SEED Role

"Chaperone protein HtpG" in subsystem Protein chaperones

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.4.10 or 5.6.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZUW2 at UniProt or InterPro

Protein Sequence (637 amino acids)

>Psyr_2017 Heat shock protein Hsp90:ATP-binding region, ATPase-like protein (Pseudomonas syringae pv. syringae B728a)
MIMSVETQKETLGFQTEVKQLLHLMIHSLYSNKEIFLRELISNASDAVDKLRFEALSKPE
LLEGGAELKIRVSFDKDAKTVTLEDNGIGMSREDVITHLGTIAKSGTADFMKNLSGDQKK
DSHLIGQFGVGFYSAFIVADQVEVFSRRAGTPASEGVHWSSKGEGEFEVATVDKADRGTR
IVLHLKNGEEEFADGYRLRNIIKKYSDHIALPIELPKEQAPAAEGEEPAALEWETVNRAS
ALWTRPRTEVKDEEYQEFYKHVAHDYENPLSWSHNKVEGKLEYTSLLYVPARAPFDLYQR
EAPRGLKLYVQRVFVMDQAESFLPLYMRFVKGVVDSNDLSLNVSREILQKDPIIDSMKSA
LTKRVLDMLEKLAKNEPEKYKGFWKNFGQVLKEGPAEDFANKEKIAGLLRFASTSDDSGE
QSVSLAEYLARAKEGQDKIYYLTGESYAQVKNSPHLEVFRKKGIEVLLLTDRIDEWLMSY
LSDFDGKGFVDVARGDLDLGNLDSEEDKKAQEEIAKDKEGLIERLKAALGESVSEVRVSH
RLTDSPAILAIGEQDMGLQMRQILEASGQKVPDSKPIFEFNPAHPLIGKLDAEQSEDRFG
DLSHILFDQAALAAGDSLKDPAAYVRRLNKLLVELSV