Protein Info for Psyr_1958 in Pseudomonas syringae pv. syringae B728a

Annotation: Non-ribosomal peptide synthase:Amino acid adenylation

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 200 400 600 800 1000 1200 1400 1600 1800 2000 2200 2400 2666 transmembrane" amino acids 291 to 310 (20 residues), see Phobius details amino acids 564 to 581 (18 residues), see Phobius details PF00668: Condensation" amino acids 50 to 484 (435 residues), 331.4 bits, see alignment E=1.7e-102 amino acids 1156 to 1588 (433 residues), 246.7 bits, see alignment E=8.7e-77 amino acids 1619 to 2040 (422 residues), 305.5 bits, see alignment E=1.2e-94 PF00501: AMP-binding" amino acids 508 to 810 (303 residues), 206.2 bits, see alignment E=1.5e-64 amino acids 869 to 916 (48 residues), 30.2 bits, see alignment (E = 4.4e-11) amino acids 2060 to 2410 (351 residues), 307.6 bits, see alignment E=2.3e-95 PF13193: AMP-binding_C" amino acids 975 to 1052 (78 residues), 28.1 bits, see alignment (E = 6.3e-10) amino acids 2468 to 2541 (74 residues), 43 bits, see alignment (E = 1.4e-14) PF00550: PP-binding" amino acids 1077 to 1137 (61 residues), 52.5 bits, see alignment (E = 9.4e-18) amino acids 2568 to 2631 (64 residues), 47.8 bits, see alignment (E = 2.8e-16) TIGR01720: non-ribosomal peptide synthase domain TIGR01720" amino acids 1456 to 1609 (154 residues), 156.9 bits, see alignment 3.7e-50 TIGR01733: amino acid adenylation domain" amino acids 2080 to 2484 (405 residues), 449.7 bits, see alignment E=8.8e-139

Best Hits

Predicted SEED Role

No annotation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZV21 at UniProt or InterPro

Protein Sequence (2666 amino acids)

>Psyr_1958 Non-ribosomal peptide synthase:Amino acid adenylation (Pseudomonas syringae pv. syringae B728a)
MTGTTAARIAKRFVGLSLEQRQQFLARLRQEGKDFSLLPVPVSRHDVSAIPLSFAQQRLL
FLWQLDPSSDAYKMTTGLRLRGTLNESALRRAFDHLIERHEVLRTVFHTDGDQAQQVLMH
DQTVALESVDLTGLADVELRDAELALQVATVTGQPFDLRTGPLLRAHLFRLAGDEHVLIV
SMHHIVSDGWSMDVMIQEFVHCYQAYCEGREAALPELPLQYADYAIWQRSWLEAGEGARQ
LDYWRNQLGEEQPLLDAAPDFPRPATQSYQGEHLRFDFGVDLSRRLNAFARTQGMTLFML
VLAGFSLFLSRKAGQRDIRIGVPNANRGRAETEGLIGFFINTQVLRCQVDERLSYLDLLA
QIRDTSFGAQAHQDVPFEQLVDQLAPERSLGHNPLFQAKFNQNVVLKQKTALRLAGLEVS
EYAFDKQGAHFDLALDITDDGTLIHGDMAYASDLYRRTTVEGFIPELLALFETLLDAPQA
PLFSLGALAIEPRREAREPAPHMLQLWDRQVERQPHAQAARSLERTLTTLQLEQAANQLA
HHLIRMGISEGQPVAVLMERSLDWLTAVLAIFKAGGVYMPLDVKAPDARLQQMLINAQAR
VLLCAEGDVRQTSLGVAGCQGLTWTPALWQDLPVSRPDTRPSADSAAYVIHTSGSTGQPK
GVLVSQGALASYLRGLLEQLHLAPEASMALVSTIAADLGHTVLFGALCSGRTLHVLTEAL
GFDPDAFAAYMAEHQVGVLKIVPGHLAALLQAGQPADVLPQHALIVGGEACSPALVEQLR
QLKPGCRVINHYGPSETTVGVLTYEVPASSCLDQKLKADSNPCGSELARESDSPAFPSRK
NVQTSSRASEASPSSLPQLPCFPQSTAHSGDPLRSVPVGTPLPGASAYVLDDVLNPVGTQ
VAGELYIGGDSVALGYIGQPALTAERFVPDPFAQDGTRVYRSGDRMRRNHQGLLEFIGRA
DDQVKVRGYRVEPAEVARVLLGLASVAQVSVLALPIDDDQARLQLVAYCVAAGGADLSID
SLREQLTACLPDYMVPAQIMLLDSLPLTANGKLDKRALPKPGVVKQRYTAPVGEIEEKLA
AVWADVLKLEQVGSTDNFFELGGDSILSLQIIARAKRQGIKLSPKQLFEKQTIGQLASVA
KLIQKKPVAVAEQISGSLPLLPIQARFFELDIPERQHWNQALMLKPLQTLEAAHLQSALT
ALVEQHDALRLGFTQQNGQWQATFGAPNTCELLWTHELDSIERLPELADEAQRSLDLKNG
PLLRAVLIHLPQGEQRLLLVIHHLLVDGVSWRVLLEDLQQAYLALAQGQPVALAAKTTSL
QRWAEQLQQYATGQTLKAERDYWLQALQGADQPLPRDKPEGTMRNRDAAHASSWLSRDLT
HKLLKVAPAAYRTHVNDLLLTALAQVLCEWSQQPSVLIQLEGHGREDLFDETDLSRTVGW
FSSLFPVRLTPQIAPGASLSGIKEQLRAVPDKGIGYGVLRYMGEASFAQQLAALPQARVT
FNYLGQFDGSFSEHQGALFVPSADSSGTALCEDGPLGNWLSLNGQVFDGQLQLDWSFSRE
VYHASTIDTLARRYEQALTTLIEHCTAGHQGVTPSDFPLARLTQAQLDGLPIAAGQIDDI
YPLSPMQQGMLFHSLFDEGAGNYINQLRVNIHGLDVPRFRSAWQAAVDHHDVLRSCFVSQ
REQSLQVVQRQVTLPFVELDARGQPESWLDDWAQADRQQGFDLAQGPLLRLAVLRTADDA
WHLVYTSHHILMDGWSSSRLLGEVLQRYSGQMPAKQAGRYRDYIQWLQHQDAALSERFWT
AQLAELDEPTRLLQAFKSSAQGGGYGDYIQLIDADGTRRLNAFAREQRVTLNTLLQSAWL
LLLQRYTGQSSVTFGATVAGRPAELPGVEEQLGLFINTLPVIASPRAEQTVADWVQQVQA
KNLALREHEHTPLYDIQRWARNSGEALFDNILVFENYPVSEALQRAPDGLVFSDLRNQEQ
AHYPLTLVVEATEVLSVRFSYDRQHFSAEGILQLAAHFDHLLQSLCASATTPLGELALPT
AWTQSVQRYPSEHCAHQRIEAQAERNPQAIALSFGDEQLSYRQLNQRANQLAHKLREQGV
GPDVRVGLAAERGLEMIVGLLAILKAGGAYVPLDPDYPQDRLSFLMHDSGIELLLTQASL
LGKLPIPEQVQMLDLADELDDYSTADPINQTAPDNLAYVIYTSGSTGKPKGTLLAHHNLM
RLFAATDEWFEFSEKDVWTLFHSFAFDFSVWEIFGALLHGGRLVIVPREVTRSPEDFHAL
LVEQQVTVLNQTPSAFKQLMHVACDSSLPMSLQTVIFGGEALDVASLKPWFARFGDQSPQ
LINMYGITETTVHVSYRPITLADTQNPASPIGEAIPDLSWYVLDADFNPVAQGCSGELHI
GHAGLARGYHNRAALTAERFVPDPFSNEGGRLYRTGDLARYRATGVIDYAGRIDHQVKIR
GFRIELGEIEARLQAHPAVREVSVLALDGQLAAYLVPAEPDQDQPTLREMLKNELRAHLP
DYMVPTHFIVLDSMPLTANGKLDRKALPAPDASQLQATYSAPQGELEQQLAAIWADLLQV
ERVGRNDNFFELGGHSLLAVQMLVRVREQLQHEVSLKDVFEQPLLADFCATLQEKNGESD
HAQDELTKSLEALKRLSAEEIDNLIA