Protein Info for Psyr_1925 in Pseudomonas syringae pv. syringae B728a

Annotation: diaminobutyrate aminotransferase apoenzyme

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 426 TIGR02407: diaminobutyrate--2-oxoglutarate aminotransferase" amino acids 8 to 412 (405 residues), 601 bits, see alignment E=9.1e-185 TIGR00709: 2,4-diaminobutyrate 4-transaminase" amino acids 9 to 417 (409 residues), 429.4 bits, see alignment E=1.3e-132 PF00202: Aminotran_3" amino acids 23 to 412 (390 residues), 254.1 bits, see alignment E=1.1e-79

Best Hits

Swiss-Prot: 53% identical to ECTB_BACHD: Diaminobutyrate--2-oxoglutarate transaminase (ectB) from Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)

KEGG orthology group: K00836, diaminobutyrate-2-oxoglutarate transaminase [EC: 2.6.1.76] (inferred from 100% identity to psb:Psyr_1925)

MetaCyc: 51% identical to diaminobutyrate-2-oxoglutarate transaminase monomer (Halomonas elongata DSM 2581)
Diaminobutyrate--2-oxoglutarate transaminase. [EC: 2.6.1.76]

Predicted SEED Role

"Diaminobutyrate-pyruvate aminotransferase (EC 2.6.1.46)" in subsystem Ectoine biosynthesis and regulation (EC 2.6.1.46)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.6.1.76

Use Curated BLAST to search for 2.6.1.46 or 2.6.1.76

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZV53 at UniProt or InterPro

Protein Sequence (426 amino acids)

>Psyr_1925 diaminobutyrate aminotransferase apoenzyme (Pseudomonas syringae pv. syringae B728a)
MNPSMAIYERESNVRSYCRDCDVVFATAQGAVIEDVEGRQFIDFFAGAGSLNYGHNNPRL
KKELLDYIQQDGITTTLDFHSAAKSRFIEQFERIILTPRGMNYKLQFTGPTGTNAIEAAL
KLARKITGRTCIAAFTHAFHGVTLGSLALTANADKRAVSGVELSNVVRLPYDGFLGRDID
SAHIIETMFSSAGAGFEPPAAIILEVVQGEGGLNCARPQWLQRIQQLCRASGALLIIDDI
QAGCGRTGTFFSFESADISPDIICLSKSISGYGLPMSLVLIKPEHDQWAPGEHNGTFRGN
NTAFVTAAAALSYWQDNAFERSIAQRSAVLRLALEKIIAHSPPEAVRIVGRGLFMGLRFG
DSRRASRLSAECLSQGLLVETCGPLNEVLKLMPPLTIDLPVLERGLDILKRAYLSTLHNE
SSRGIA