Protein Info for Psyr_1812 in Pseudomonas syringae pv. syringae B728a

Annotation: guanine deaminase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02967: guanine deaminase
  • amino acids 38 to 437 (400 residues), 625.2 bits, E=2.4e-192
• PF01979: Amidohydro_1
  • amino acids 79 to 440 (362 residues), 218.2 bits, E=1.9e-68
• PF07969: Amidohydro_3
  • amino acids 279 to 441 (163 residues), 49.3 bits, E=5.8e-17

Best Hits

Swiss-Prot: 57% identical to GUAD_ECOLI: Guanine deaminase (guaD) from Escherichia coli (strain K12)

KEGG orthology group: K01487, guanine deaminase [EC: 3.5.4.3] (inferred from 100% identity to psb:Psyr_1812)

MetaCyc: 57% identical to guanine deaminase (Escherichia coli K-12 substr. MG1655)
Guanine deaminase. [EC: 3.5.4.3]

Predicted SEED Role

"Guanine deaminase (EC 3.5.4.3)" in subsystem Purine Utilization or Purine conversions (EC 3.5.4.3)

MetaCyc Pathways

• purine nucleotides degradation I (plants) (10/12 steps found)
• guanosine nucleotides degradation II (4/4 steps found)
• purine nucleotides degradation II (aerobic) (9/11 steps found)
• superpathway of purines degradation in plants (14/18 steps found)
• superpathway of guanosine nucleotides degradation (plants) (5/6 steps found)
• guanosine nucleotides degradation III (3/4 steps found)
• drosopterin and aurodrosopterin biosynthesis (5/7 steps found)
• purine nucleobases degradation II (anaerobic) (16/24 steps found)
• purine nucleobases degradation I (anaerobic) (6/15 steps found)

KEGG Metabolic Maps

• Purine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.5.4.3

Use Curated BLAST to search for 3.5.4.3

Sequence Analysis Tools

See Q4ZVG3 at UniProt or InterPro

Protein Sequence (444 amino acids)

>Psyr_1812 guanine deaminase (Pseudomonas syringae pv. syringae B728a)
MSHNESAMSSVSRRKAYRAAIVHSLADPAEVALEASCEYFADGLLLVEDGKIVSVGHAAD
LLGRLDDDVELIEYTDALITPGFIDTHIHLPQTGMIGSYGEQLLDWLNTYTFPCESQFAD
PAHSAQVADIFIKELLRNGTTTALVFGSVHKESVEAFFSAAQALDLRMIAGKVMMDRNAP
DYLVDTPESGYADSKALIERWHGKGRLSYAVTPRFAPTSSPEQLSLAGQLLTEYPGLYLQ
THISENLQEIEWVKALFPERKHYLDVYDHFNLLGERSVFAHGVHLCDEQCARLAQAGSAI
AFCPTSNLFLGSGLFNLPMAEKHKVNVGLGTDVGGGTSFSILQTLNEAYKVMQMQGARLN
PFKSLYLATLGGARALRLEDKVGSLKPGNEADFLVLDYNATPLLSYRLKQAKTIEEILFV
LMTIGDDRTVKQTWSGGRLVHDRG