Protein Info for Psyr_1801 in Pseudomonas syringae pv. syringae B728a

Annotation: 1-aminocyclopropane-1-carboxylate deaminase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 100% identical to 1A1D_PSEU2: 1-aminocyclopropane-1-carboxylate deaminase (acdS) from Pseudomonas syringae pv. syringae (strain B728a)

KEGG orthology group: K01505, 1-aminocyclopropane-1-carboxylate deaminase [EC: 3.5.99.7] (inferred from 99% identity to psp:PSPPH_1761)

Predicted SEED Role

"1-aminocyclopropane-1-carboxylate deaminase (EC 3.5.99.7)" (EC 3.5.99.7)

MetaCyc Pathways

superpathway of branched chain amino acid biosynthesis (17/17 steps found)
superpathway of L-isoleucine biosynthesis I (13/13 steps found)
L-isoleucine biosynthesis I (from threonine) (7/7 steps found)
L-methionine degradation II (2/3 steps found)
L-threonine degradation I (4/6 steps found)
superpathway of L-threonine metabolism (11/18 steps found)
hypoglycin biosynthesis (4/14 steps found)

KEGG Metabolic Maps

Propanoate metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.5.99.7

Use Curated BLAST to search for 3.5.99.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZVH4 at UniProt or InterPro

Protein Sequence (338 amino acids)

>Psyr_1801 1-aminocyclopropane-1-carboxylate deaminase (Pseudomonas syringae pv. syringae B728a)
MNLSKFKRYPLTFGPSPITPLKRLSEHLGGKVELYAKREDCNSGLAFGGNKTRKLEYLVP
EAIDGGYDTLVSIGGIQSNQTRQVAAVAAHLGMKCVLVQENWVNYSDALYDRVGNIEMSR
IMGADVRLDSAGFDIGIRPSWEKAMADVEESGGKPFPIPAGCSEHPYGGLGFVRFADEVR
QQEEELGFKFDYIVVCSVTGSTHAGMLVGFAADGRAQRVIGIDASAKPEKTRAQVLRIAQ
NTAKLVELGREITAEDVVLDTRYAYPEYGLPNDGTLEAIRLCARLEGVLTDPVYEGKSMH
GMIDMVRNGEFPEGSKVLYAHLGGVPALNAYSFLFKDG