Protein Info for Psyr_1698 in Pseudomonas syringae pv. syringae B728a

Annotation: Glutathionylspermidine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF03738: GSP_synth
  • amino acids 12 to 383 (372 residues), 484.4 bits, E=1.3e-149

Best Hits

Swiss-Prot: 46% identical to YJFC_ECOLI: Putative acid--amine ligase YjfC (yjfC) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to psb:Psyr_1698)

MetaCyc: 46% identical to peptide--spermidine ligase YjfC (Escherichia coli K-12 substr. MG1655)
6.3.1.-

Predicted SEED Role

"Similarity with glutathionylspermidine synthase (EC 6.3.1.8), group 1" in subsystem Glutathionylspermidine and Trypanothione (EC 6.3.1.8)

MetaCyc Pathways

• glutathionylspermidine biosynthesis (1/1 steps found)

KEGG Metabolic Maps

• Glutathione metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.1.8

Sequence Analysis Tools

See Q4ZVS6 at UniProt or InterPro

Protein Sequence (385 amino acids)

>Psyr_1698 Glutathionylspermidine synthase (Pseudomonas syringae pv. syringae B728a)
MKKISIEERPDWRTTAEREGFNFHTIDGERYWDERGYYLFSEQQITRDLEAPTQELHQMC
MDAVARVVDSEALLHQLAIPEAFFDLIQSSWKEGHPHLYGRFDFAYDGTGPAKMYEINAD
TPTSLMEAGAVQLLWLEEQMARGVLPAHATQFNSIAEDLVRAFAEFPGTTPFYFSAMSGS
IEDRGTTDFLRRMAAHVGIDARHIDIEDIGLGADGRFVDLDGRWIERLFKLHAWEHIFHE
EFGQHVSASGTQFVEPAWKAILSNKGILPLLWQFNEGHPNLLPAHVDQDANKAVPKGWVR
KPYFSREGANIEMRTPGDQVISVDGPYTDAPYILQAYSPLPRFGDSYTLIGSWVIGDLAS
GIGIREDDSLITKDTSRFLPHVVID