Protein Info for Psyr_1478 in Pseudomonas syringae pv. syringae B728a

Annotation: regulatory protein, LysR:LysR, substrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 347 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF00126: HTH_1" amino acids 5 to 63 (59 residues), 62.4 bits, see alignment E=3.2e-21 PF03466: LysR_substrate" amino acids 89 to 291 (203 residues), 163.2 bits, see alignment E=5.8e-52

Best Hits

Swiss-Prot: 46% identical to YEIE_ECOLI: Uncharacterized HTH-type transcriptional regulator YeiE (yeiE) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to psb:Psyr_1478)

Predicted SEED Role

"LysR family transcriptional regulator YeiE" in subsystem YeiH

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZWE4 at UniProt or InterPro

Protein Sequence (347 amino acids)

>Psyr_1478 regulatory protein, LysR:LysR, substrate-binding protein (Pseudomonas syringae pv. syringae B728a)
MKLSLRQLQIFCAVAQHGSTTSAAISVSLSQSATSAALNELESALDTRLFDRVGKRLVLN
DNGHALLPQARRMLENAQQIEASFLPGHAGLKTRLRVGCSTTIGNYVLPLILGELRQSTP
HLRVDVDMANSMAIARRVADFEIDMGLIEGPCHLPELSAEPWLIDELLIAAGQRHPLALK
QQVTLDDLRTAEWLLREPGSGTREEVEQLLLRHLHDLAETRQIGSSEAIKHTLAQGIGIS
CLSRWVVRDLLASEVLVELPHALPALTRRFFLIRHRDKYISPGLERFWEKCSQFVSQEQL
DLFIKQVRATKTSCFRNLSTTHKVNSLPCSDERVVYAKARLGALLTL