Protein Info for Psyr_1357 in Pseudomonas syringae pv. syringae B728a
Annotation: RNase HII
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to RNH2_PSEU2: Ribonuclease HII (rnhB) from Pseudomonas syringae pv. syringae (strain B728a)
KEGG orthology group: K03470, ribonuclease HII [EC: 3.1.26.4] (inferred from 100% identity to psb:Psyr_1357)MetaCyc: 71% identical to RNase HII (Escherichia coli K-12 substr. MG1655)
Ribonuclease H. [EC: 3.1.26.4]
Predicted SEED Role
"Ribonuclease HII (EC 3.1.26.4)" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase or DNA replication, archaeal or Ribonuclease H (EC 3.1.26.4)
Isozymes
Compare fitness of predicted isozymes for: 3.1.26.4
Use Curated BLAST to search for 3.1.26.4
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q4ZWR4 at UniProt or InterPro
Protein Sequence (218 amino acids)
>Psyr_1357 RNase HII (Pseudomonas syringae pv. syringae B728a) MMQTGLDFTLVEDLVAGVDEVGRGPLCGAVVTAAVILDPTRPILGLNDSKKLTEARREKL YVEIQEKALCWFIARAEVEEIDQLNILHATMLAMQRAVEGLSITPRLALIDGNRCPQLSV PSAPVVKGDSKVPAIAAASILAKVSRDREMAAFELIYPGYGIGGHKGYPTPVHLEALARL GPTPIHRRSFAPVRAAHEARATIMMGGSISPSVGLLQD