Protein Info for Psyr_1341 in Pseudomonas syringae pv. syringae B728a

Annotation: Protein-P-II uridylyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 898 PF03445: DUF294" amino acids 34 to 129 (96 residues), 24.1 bits, see alignment E=1.2e-08 TIGR01693: protein-P-II uridylyltransferase" amino acids 36 to 884 (849 residues), 1095.6 bits, see alignment E=0 PF01909: NTP_transf_2" amino acids 67 to 117 (51 residues), 31.4 bits, see alignment 8.5e-11 PF08335: GlnD_UR_UTase" amino acids 185 to 322 (138 residues), 154.3 bits, see alignment E=8.5e-49 PF27444: GlnD_3rd" amino acids 346 to 439 (94 residues), 119.9 bits, see alignment E=1.9e-38 PF01966: HD" amino acids 486 to 559 (74 residues), 34.7 bits, see alignment E=7.9e-12 PF27446: GlnD_5th" amino acids 626 to 677 (52 residues), 70 bits, see alignment 5.5e-23 PF01842: ACT" amino acids 814 to 863 (50 residues), 30.4 bits, see alignment 1e-10

Best Hits

Swiss-Prot: 100% identical to GLND_PSEU2: Bifunctional uridylyltransferase/uridylyl-removing enzyme (glnD) from Pseudomonas syringae pv. syringae (strain B728a)

KEGG orthology group: K00990, [protein-PII] uridylyltransferase [EC: 2.7.7.59] (inferred from 100% identity to psb:Psyr_1341)

Predicted SEED Role

"[Protein-PII] uridylyltransferase (EC 2.7.7.59)" in subsystem Ammonia assimilation (EC 2.7.7.59)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.59

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZWT0 at UniProt or InterPro

Protein Sequence (898 amino acids)

>Psyr_1341 Protein-P-II uridylyltransferase (Pseudomonas syringae pv. syringae B728a)
MPQVDPDLFDRGQFQAELALKASPIAAFKKAIRRARDVLDERFRSGRDIRRLIEDRAWFV
DNILQKAWDQFEWSEDADIALLAVGGYGRGELHPYSDIDLLILLDSDDHEVFREPIERFL
TLLWDIGLEVGQSVRSVNECAQEGRADLTVITNLMESRTIAGPEHLRQRMLEVTSTEHMW
PSKEFFLAKHAEQKKRHHKYNDTEYNLEPNVKGSPGGLRDIQTILWVARRQYGTLNLHAL
AGEGFLLGSENALLASSQEFLWKVRYALHMLAGRSEDRLLFDYQVRIAGLLGYEDNDAKL
AIERFMQKYYRVVMSIAELSDLIIQHFEEVILSDDSGTPQPINSRFQLHDGYIEATHQNV
FKRTPFAMIEIFVLMAQHPEIKGVRADTIRLLREHRHLINDEFRNDIRNTSLFIELFKCE
IGIHRNLRRMNRYGILGLYLPEFGHIVGQMQHDLFHIYTVDAHTLNLIKHLRKLQYTEVS
EKFPLASKIMGRLPKPELIYLAGLYHDIGKGRGGDHSELGAIDAQAFGARHHLPAWDTRL
IVWLVSNHLVMSTTAQRKDLSDPQVIHDFAQFVGDEVHLDYLYVLTVADINATNPTLWNS
WRASLLRQLYTETKRALRRGLENPVDREEQIRRTQTAALDILVRNGTDPDDVEQLWSALG
DDYFLRHTAGDVAWHSDAILQQPADGGPLVLIKETTQREFEGGTQIFIYAPDQHDFFAVT
VAAMDQLNLNIHDARIITSSSKFTLDTYIVLDHEGGSIGNNPERIQDIREGLTEALRNPD
DYPTIIKRRVPRQLKHFAFAPQVTIHNDAQRPVTVLELLAPDRPGLLARIGKIFLEFDLS
LQNAKIATLGERVEDVFFITDANNHPLSDPQLCRQLQDAIVKQLSVNSEPGNDLRISI