Protein Info for Psyr_1269 in Pseudomonas syringae pv. syringae B728a

Annotation: phosphoribosylformylglycinamidine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01735: phosphoribosylformylglycinamidine synthase
  • amino acids 3 to 1297 (1295 residues), 2121.4 bits, E=0
• PF18076: FGAR-AT_N
  • amino acids 35 to 148 (114 residues), 139.9 bits, E=8.8e-45
• PF18072: FGAR-AT_linker
  • amino acids 169 to 218 (50 residues), 78.4 bits, see alignment (E = 1.2e-25)
• PF02769: AIRS_C
  • amino acids 430 to 587 (158 residues), 116.6 bits, E=3.1e-37
  • amino acids 839 to 968 (130 residues), 51.7 bits, E=2.9e-17
• PF22689: FGAR-AT_PurM_N-like
  • amino acids 646 to 805 (160 residues), 283.7 bits, E=9.1e-89
• PF13507: GATase_5
  • amino acids 1042 to 1297 (256 residues), 363.1 bits, E=1.7e-112

Best Hits

Swiss-Prot: 66% identical to PUR4_IDILO: Phosphoribosylformylglycinamidine synthase (purL) from Idiomarina loihiensis (strain ATCC BAA-735 / DSM 15497 / L2-TR)

KEGG orthology group: K01952, phosphoribosylformylglycinamidine synthase [EC: 6.3.5.3] (inferred from 62% identity to aap:NT05HA_1480)

MetaCyc: 65% identical to phosphoribosylformylglycinamide synthetase (Escherichia coli K-12 substr. MG1655)
Phosphoribosylformylglycinamidine synthase. [EC: 6.3.5.3]

Predicted SEED Role

"Phosphoribosylformylglycinamidine synthase, synthetase subunit (EC 6.3.5.3) / Phosphoribosylformylglycinamidine synthase, glutamine amidotransferase subunit (EC 6.3.5.3)" in subsystem De Novo Purine Biosynthesis (EC 6.3.5.3)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (24/26 steps found)
• superpathway of 5-aminoimidazole ribonucleotide biosynthesis (6/6 steps found)
• 5-aminoimidazole ribonucleotide biosynthesis I (5/5 steps found)
• 5-aminoimidazole ribonucleotide biosynthesis II (5/5 steps found)

KEGG Metabolic Maps

• Purine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.5.3

Sequence Analysis Tools

See Q4ZX02 at UniProt or InterPro

Protein Sequence (1298 amino acids)

>Psyr_1269 phosphoribosylformylglycinamidine synthase (Pseudomonas syringae pv. syringae B728a)
MLILRGAPALSAFRHSKLLEQLKQKVSAVSGLYAEFAHFADVNDVLTSEEQQVLDRLLKY
GPSVPVQEPSGRLFLVLPRFGTISPWSSKASDIARNCGLSKIQRLERGIAFYVEGQFSET
EAQAIADSLHDRMTQLVLGDLEQAANLFSHAQPKPLTAVDILGGGRAALEKANVELGLAL
AEDEIDYLITSFNGLGRNPHDIELMMFAQANSEHCRHKIFNASWDIDGQSQEKSLFGMIK
NTYQMHSEGVLSAYKDNASVIVGNVAGRFFPDPETRQYGAVQEPVHILMKVETHNHPTAI
APFPGAATGSGGEIRDEGATGRGAKPKAGLTGFTVSNLQIPGFVQPWEVPYGKPERIVTA
LDIMIEGPLGGAAFNNEFGRPALTGYFRTFEQSITTPHGDEVRGYHKPIMLAGGMGNIRE
DHVQKGEITVGSKLIVLGGPAMLIGLGGGAASSMATGTSSADLDFASVQRENPEMERRCQ
EVIDRCWQLGDRNPISFIHDVGAGGLSNAFPELVNDGDRGGRFELRNVPNDEPGMAPLEI
WSNESQERYVLAVGVEDFERFKAICERERCPFAVVGEATAEPQLTVTDSHFGNSPVDMPL
EVLLGKAPRMHRSVAREEEIGDDFDPSTLDIEESVQRVLRHPAVASKSFLITIGDRSITG
LVARDQMVGPWQVPVADCAVTATSFDVNTGEAMAMGERTPLALLDAPASGRMAIGETLTN
IAASLIEKLSDIKLSANWMSAAGHPGEDARLYDTVKAVGMELCPELGITIPVGKDSMSMK
TRWSDEGTEKSVTSPLSLIVTGFAPVVDIRQTLTPELRMDKGITDLILIDLGRGQNRMGA
SILAQTHGKLGRVAPDVDDAEDLKAFFAVIQGLNSDGHILSYHDRSDGGLLVSTLEMAFA
GHCGLNLHLDGVADNVSELSAILFNEELGAVIQVRQDATPLVLAQFSAAGLEDCVAVIGQ
PINNDEVSISFHGEPVFSGQRRLLQRQWAETSYQIQRLRDNAECADQEFDALLEEDNPGL
TVKLGFDVNDDIAAPYIKTGVRPQVAVLREQGVNGQVEMAAAFDRAGFNAIDVHMSDILA
GRVDLNDFKGMVACGGFSYGDVLGAGEGWAKSALFNSRARDAFQGFFERSDSFTLGVCNG
CQMLSNLHELIPGSEFWPHFVRNRSEQFEARVAMVQVQESASIFLQGMAGSRMPIAIAHG
EGHAEFRNDDALLEADVSGTVALRFVDNHGKVTETYPANPNGSPRGIGGMTTLDGRVTIM
MPHPERVFRAVQNSWRPEDWNEDGAWMRMFRNARAWVN