Protein Info for Psyr_1259 in Pseudomonas syringae pv. syringae B728a

Annotation: Exodeoxyribonuclease VII large subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 463 PF13742: tRNA_anti_2" amino acids 20 to 112 (93 residues), 103.6 bits, see alignment E=8.2e-34 TIGR00237: exodeoxyribonuclease VII, large subunit" amino acids 21 to 364 (344 residues), 433.8 bits, see alignment E=3.3e-134 PF01336: tRNA_anti-codon" amino acids 40 to 114 (75 residues), 51.6 bits, see alignment E=1.1e-17 PF02601: Exonuc_VII_L" amino acids 136 to 447 (312 residues), 368.5 bits, see alignment E=5.5e-114

Best Hits

Swiss-Prot: 100% identical to EX7L_PSEU2: Exodeoxyribonuclease 7 large subunit (xseA) from Pseudomonas syringae pv. syringae (strain B728a)

KEGG orthology group: K03601, exodeoxyribonuclease VII large subunit [EC: 3.1.11.6] (inferred from 100% identity to psb:Psyr_1259)

Predicted SEED Role

"Exodeoxyribonuclease VII large subunit (EC 3.1.11.6)" in subsystem DNA repair, bacterial (EC 3.1.11.6)

Isozymes

Compare fitness of predicted isozymes for: 3.1.11.6

Use Curated BLAST to search for 3.1.11.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZX12 at UniProt or InterPro

Protein Sequence (463 amino acids)

>Psyr_1259 Exodeoxyribonuclease VII large subunit (Pseudomonas syringae pv. syringae B728a)
MMPDMIKDPFARLGLDREVLTVSQLNGRARVLLEDVFSSIWVEGEISNLSRPASGHVYFT
LKDSGAQVRCALFRQSAARVRQALKDGLQVKVRGKVSLFEGRGDYQLILDTVEPAGDGAL
RLAFDALKAKLSDEGLFSAERKVALPLHPQRIGIISSPTGAVIRDIISVFRRRAPRVELT
LIPTAVQGREAINQIVRALKLADSRGFDALILARGGGSLEDLWCFNEEAVARAIDACVTP
IVSAVGHETDVSISDFVADVRAPTPSAAAELLAPDSSDLHRRVDNLHRRLVSRMQDRLMR
ERLRLEGISRRLRHPGERLRQQSQRLDDLDMRLRRALEQTMHQRQLRLAHMQSRLAAQHP
GRTLAFLRQRLDALAERLPRAIREQIKARKLQLQSQVQTLNVVSPLATLGRGYSILLDER
GHAIRNAAQTQTGQRLTARLGEGELHVRVEDNHLTPVTLSLLD