Protein Info for Psyr_1217 in Pseudomonas syringae pv. syringae B728a

Annotation: Sigma-70 region 2:Sigma-70 region 4

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 184 TIGR02937: RNA polymerase sigma factor, sigma-70 family" amino acids 23 to 179 (157 residues), 71.5 bits, see alignment E=3.3e-24 PF04542: Sigma70_r2" amino acids 31 to 89 (59 residues), 40.9 bits, see alignment E=2.1e-14 PF08281: Sigma70_r4_2" amino acids 125 to 176 (52 residues), 36.5 bits, see alignment E=4.7e-13 PF04545: Sigma70_r4" amino acids 129 to 178 (50 residues), 38.2 bits, see alignment E=1.3e-13

Best Hits

Swiss-Prot: 97% identical to HRPL_PSESY: RNA polymerase sigma factor HrpL (hrpL) from Pseudomonas syringae pv. syringae

KEGG orthology group: None (inferred from 100% identity to psb:Psyr_1217)

Predicted SEED Role

"RNA polymerase sigma factor RpoE" in subsystem Transcription initiation, bacterial sigma factors

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZX51 at UniProt or InterPro

Protein Sequence (184 amino acids)

>Psyr_1217 Sigma-70 region 2:Sigma-70 region 4 (Pseudomonas syringae pv. syringae B728a)
MLPNLVILDATEPRQPSSSAGIRQLTADQVQMLRAFIQKRVKNADDVDDILQCVFLEALR
NEHKFQHASKPQTWLCGIALNLIRNHFRKMYRQPYQESWEDEVHTDLEWNGDITHQVDGH
RQLARVIEAIDCLPTNMQKVLEVSLEMDGNYQETANTLGVPIGTVRSRLSRARVQLKQHI
DPFA