Protein Info for Psyr_1165 in Pseudomonas syringae pv. syringae B728a

Annotation: Isochorismatase hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

KEGG orthology group: None (inferred from 100% identity to psb:Psyr_1165)

MetaCyc: 74% identical to biuret amidohydrolase subunit (Rhizobium leguminosarum bv. viciae 3841)
Biuret amidohydrolase. [EC: 3.5.1.84]

Predicted SEED Role

No annotation

MetaCyc Pathways

cyanuric acid degradation II (4/5 steps found)
superpathway of atrazine degradation (4/8 steps found)

KEGG Metabolic Maps

Atrazine degradation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.1.84

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZXA3 at UniProt or InterPro

Protein Sequence (228 amino acids)

>Psyr_1165 Isochorismatase hydrolase (Pseudomonas syringae pv. syringae B728a)
MSERHIASAPYPWPWNGQLHAHNTALIVIDMQTDFCGVGGYVDSMGYDLALTRAPIEPIK
ALLATMRPLGFTIIHTREGHRPDLSDLPANKRWRSQRIGAGIGDPGPCGKILVRGEPGWE
IIDELAPLPGEIVLDKPGKGSFCATDLELILRTRGIDNLILTGITTDVCVHTTLREANDR
GFECLLLEDCCGATDPDNHAAALSMVKMQGGVFGAVGHSSMLRDLLGA