Protein Info for Psyr_1148 in Pseudomonas syringae pv. syringae B728a

Annotation: branched chain amino acid aminotransferase apoenzyme

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 70% identical to ILVE_HAEIN: Branched-chain-amino-acid aminotransferase (ilvE) from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

KEGG orthology group: K00826, branched-chain amino acid aminotransferase [EC: 2.6.1.42] (inferred from 100% identity to psb:Psyr_1148)

MetaCyc: 38% identical to branched-chain amino acid aminotransferase (Bacillus subtilis subtilis 168)
Branched-chain-amino-acid transaminase. [EC: 2.6.1.42, 2.6.1.6]; 2.6.1.42 [EC: 2.6.1.42, 2.6.1.6]; 2.6.1.42 [EC: 2.6.1.42, 2.6.1.6]

Predicted SEED Role

"Branched-chain amino acid aminotransferase (EC 2.6.1.42)" in subsystem Alanine biosynthesis or Branched-Chain Amino Acid Biosynthesis or Isoleucine degradation or Leucine Biosynthesis or Leucine Degradation and HMG-CoA Metabolism or Pyruvate Alanine Serine Interconversions or Valine degradation (EC 2.6.1.42)

MetaCyc Pathways

anteiso-branched-chain fatty acid biosynthesis (30/34 steps found)
even iso-branched-chain fatty acid biosynthesis (30/34 steps found)
odd iso-branched-chain fatty acid biosynthesis (30/34 steps found)
superpathway of branched chain amino acid biosynthesis (17/17 steps found)
superpathway of L-isoleucine biosynthesis I (13/13 steps found)
L-isoleucine biosynthesis I (from threonine) (7/7 steps found)
L-leucine biosynthesis (6/6 steps found)
L-valine biosynthesis (4/4 steps found)
superpathway of L-alanine biosynthesis (4/4 steps found)
L-leucine degradation I (5/6 steps found)
L-alanine biosynthesis I (2/2 steps found)
L-valine degradation I (6/8 steps found)
L-isoleucine biosynthesis V (2/3 steps found)
L-isoleucine degradation II (2/3 steps found)
L-leucine degradation III (2/3 steps found)
L-valine degradation II (2/3 steps found)
L-isoleucine biosynthesis IV (4/6 steps found)
L-isoleucine degradation I (4/6 steps found)
L-isoleucine biosynthesis III (4/7 steps found)
L-isoleucine degradation III (oxidative Stickland reaction) (1/3 steps found)
L-leucine degradation V (oxidative Stickland reaction) (1/3 steps found)
L-valine degradation III (oxidative Stickland reaction) (1/3 steps found)
L-isoleucine biosynthesis II (4/8 steps found)
superpathway of L-threonine metabolism (11/18 steps found)
L-leucine degradation IV (reductive Stickland reaction) (1/5 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.6.1.42 or 2.6.1.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZXC0 at UniProt or InterPro

Protein Sequence (339 amino acids)

>Psyr_1148 branched chain amino acid aminotransferase apoenzyme (Pseudomonas syringae pv. syringae B728a)
MSQESINWDTLGFDYIKTDKRYLSHWRDGAWDQGSLTEDNTLHISEGSTALHYGQQCFEG
LKAYRCKDGSINLFRPDQNAQRMQRSCSRLLMPHVPTDVFIEACKQVVKANERFIPPYGS
GGALYLRPFVIGVGDNIGVRTAPEFIFSIFCIPVGAYFKGGLKPNNFVISGYDRAAPNGT
GAAKVGGNYAASLMPGSEAKKHSFADCIYLDPQTHSKIEEVGSANFFAITKDDVFLTPKS
PSVLPGITRLSLIELAKSRLGLTVEEGDVFIDQLGEFKEAGACGTAAVITPIGGISYKDN
LHVFYSQTEVGPVTQRLYKELTGVQSGDIEAPAGWIVKV