Protein Info for Psyr_1062 in Pseudomonas syringae pv. syringae B728a

Annotation: alginate biosynthesis protein Alg8

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 493 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details transmembrane" amino acids 27 to 28 (2 residues), see Phobius details amino acids 47 to 70 (24 residues), see Phobius details amino acids 364 to 366 (3 residues), see Phobius details amino acids 371 to 390 (20 residues), see Phobius details amino acids 394 to 415 (22 residues), see Phobius details amino acids 426 to 444 (19 residues), see Phobius details amino acids 471 to 492 (22 residues), see Phobius details PF13641: Glyco_tranf_2_3" amino acids 86 to 342 (257 residues), 136.8 bits, see alignment E=1.1e-43 PF13632: Glyco_trans_2_3" amino acids 183 to 409 (227 residues), 96.3 bits, see alignment E=2.2e-31

Best Hits

Swiss-Prot: 98% identical to ALG8_PSESM: Glycosyltransferase alg8 (alg8) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: None (inferred from 100% identity to psp:PSPPH_1117)

MetaCyc: 82% identical to mannuronosyl transferase catalytic subunit (Pseudomonas aeruginosa)
Alginate synthase. [EC: 2.4.1.33]

Predicted SEED Role

"Alginate biosynthesis protein Alg8" in subsystem Alginate metabolism

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.33

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZXK4 at UniProt or InterPro

Protein Sequence (493 amino acids)

>Psyr_1062 alginate biosynthesis protein Alg8 (Pseudomonas syringae pv. syringae B728a)
MQRLKHGLLQAAGWLFYLSLLMGLAAALPTSIFDSQSKNFIFLIGAVGIWRYSMGITHFV
RGMIFLYIVYPHLRRKVRKLGSAADPSHVFLMVTSFRIDALTTAQVYNSVIREAIDCGLP
TTIVCSLVEMSDELLVKSMWAKFNPPERVKLDFVRIPGTGKRDGLAYGFRAISRHLPDSR
AVVAVIDGDTVLNEGVVAKTVPWFQLFDNVGGLTTNEFCEVRGGYIMSEWHKLRFAQRHI
NMCSMALSKRVLTMTGRMSVFRATVVTDPEFIADVESDSLNHWRLGTFRFLTGDDKSSWF
SLMRLGYDTFYVPDAAINTVEHPPEKSFLKASRKLMYRWYGNNLRQNSRALGLGVRRLGI
FTSIVLFDQRVSMWTSILGLTVALIASFKYGGAFLLMYLLWIGMTRLILTLLLSLSGHRI
GPAYPIILYYNQIVGALMKIYVFFRLDRQSWTRQDTKLSRDMASFQGWFNTWSSRTMTFS
AGTIFVAVLLTMV