Protein Info for Psyr_0976 in Pseudomonas syringae pv. syringae B728a

Annotation: Malate:quinone-oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 94% identical to MQO_PSESM: Probable malate:quinone oxidoreductase (mqo) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: K00116, malate dehydrogenase (quinone) [EC: 1.1.5.4] (inferred from 100% identity to psb:Psyr_0976)

MetaCyc: 54% identical to malate:quinone oxidoreductase (Escherichia coli K-12 substr. MG1655)
MALATE-DEHYDROGENASE-ACCEPTOR-RXN [EC: 1.1.5.4]

Predicted SEED Role

"Malate:quinone oxidoreductase (EC 1.1.5.4)" in subsystem TCA Cycle (EC 1.1.5.4)

MetaCyc Pathways

superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (23/26 steps found)
superpathway of glyoxylate bypass and TCA (12/12 steps found)
TCA cycle I (prokaryotic) (10/10 steps found)
partial TCA cycle (obligate autotrophs) (8/8 steps found)
TCA cycle VII (acetate-producers) (8/9 steps found)
TCA cycle VI (Helicobacter) (7/9 steps found)

KEGG Metabolic Maps

Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.5.4

Use Curated BLAST to search for 1.1.5.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZXU0 at UniProt or InterPro

Protein Sequence (502 amino acids)

>Psyr_0976 Malate:quinone-oxidoreductase (Pseudomonas syringae pv. syringae B728a)
MAHNEAVDVVLVGAGIMSATLAVLLKELDPGITLEVVELMDSGAAESSNPWNNAGTGHAG
LCELNYTPPAADGSIDIKKAVHINTQFEVSKQFWAYLARKGTFGSARSFINPVPHLSFVQ
GEKGVSFLKTRFAAMSKHHAFSSMEYTEDKAKLAEWMPLMMPGRPADEKIAATRMMNGTD
VNFGALTNQLLGHLTSAPGTQIKYRSRVTNLTRNGAGWTVSVKDVNGGGTREIDAKFVFL
GAGGAALPLLQLSGIEESKGFGGFPVSGQWLRCDNPEVVKHHQAKVYSQAAVGSPPMSVP
HLDTRVVDGKKSLLFGPYAGFTTKFLKHGSYLDLPLSVRVANIKPMLAVARDNMDLTKYL
VSEVMQSMEQRLESLRRFYPEAKAEDWRLEIAGQRVQIIKKDAKKGGVLQFGTELVSAQD
GSLAALLGASPGASVTVSIMLELLERCFPEKTRTEWATKLEEIFPAREKILETDAPLYER
ISAQNDEALELVEKSSQEHSFA