Protein Info for Psyr_0969 in Pseudomonas syringae pv. syringae B728a

Annotation: uracil phosphoribosyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 212 TIGR01091: uracil phosphoribosyltransferase" amino acids 3 to 208 (206 residues), 290.9 bits, see alignment E=2.6e-91 PF14681: UPRTase" amino acids 7 to 207 (201 residues), 224.8 bits, see alignment E=8.4e-71 PF00156: Pribosyltran" amino acids 55 to 171 (117 residues), 54.9 bits, see alignment E=6.4e-19

Best Hits

Swiss-Prot: 100% identical to UPP_PSEU2: Uracil phosphoribosyltransferase (upp) from Pseudomonas syringae pv. syringae (strain B728a)

KEGG orthology group: K00761, uracil phosphoribosyltransferase [EC: 2.4.2.9] (inferred from 99% identity to pst:PSPTO_1130)

MetaCyc: 72% identical to uracil phosphoribosyltransferase (Escherichia coli K-12 substr. MG1655)
Uracil phosphoribosyltransferase. [EC: 2.4.2.9]

Predicted SEED Role

"Uracil phosphoribosyltransferase (EC 2.4.2.9)" in subsystem De Novo Pyrimidine Synthesis or LMPTP YwlE cluster (EC 2.4.2.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.2.9

Use Curated BLAST to search for 2.4.2.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZXU7 at UniProt or InterPro

Protein Sequence (212 amino acids)

>Psyr_0969 uracil phosphoribosyltransferase (Pseudomonas syringae pv. syringae B728a)
MPIREIRHPLIRHKLGLMRRADISTKNFRELAQEVGALLTYEATADLTLENYDIQGWAGT
VSVEKIAGKKITVVPILRAGIGMLDGVLSLIPGAKVSAVGVARNEETLQAHTYLEKLVPE
IDERLAMIIDPMLATGSSMVATIDLLKKAGCKEIRAMVLVAAPEGIAAVEKAHPDVMIYT
ASIDERLNEHGYIIPGLGDAGDKIFGTKQKDA