Protein Info for Psyr_0941 in Pseudomonas syringae pv. syringae B728a

Annotation: Conserved hypothetical protein 92

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00092: GTP-binding protein YchF
  • amino acids 1 to 366 (366 residues), 524.2 bits, E=9.7e-162
• PF01926: MMR_HSR1
  • amino acids 5 to 110 (106 residues), 72.9 bits, E=3.6e-24
• PF02421: FeoB_N
  • amino acids 5 to 44 (40 residues), 29.2 bits, 9.7e-11
• PF06071: YchF-GTPase_C
  • amino acids 282 to 365 (84 residues), 138.4 bits, E=1e-44

Best Hits

Swiss-Prot: 73% identical to YCHF_HAEDU: Ribosome-binding ATPase YchF (ychF) from Haemophilus ducreyi (strain 35000HP / ATCC 700724)

KEGG orthology group: K06942, (no description) (inferred from 100% identity to psp:PSPPH_0988)

MetaCyc: 70% identical to redox-responsive ATPase YchF (Escherichia coli K-12 substr. MG1655)
Nucleoside-triphosphatase. [EC: 3.6.1.15, 3.6.1.5]

Predicted SEED Role

"GTP-binding and nucleic acid-binding protein YchF" in subsystem Universal GTPases

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (12/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5

Sequence Analysis Tools

See Q4ZXX5 at UniProt or InterPro

Protein Sequence (366 amino acids)

>Psyr_0941 Conserved hypothetical protein 92 (Pseudomonas syringae pv. syringae B728a)
MGFNCGIVGLPNVGKSTLFNALTKSGIAAENFPFCTIEPNSGIVPMPDPRLAALAAIVNP
KRILPTTMEFVDIAGLVAGASKGEGLGNKFLANIRETDAIAHVVRCFEDENVIHVSNSVD
PKRDIEIIDLELIFADLDSCEKQLQKVTRNAKGGDKDAVVQKGLLEQLIAHFSEGKPARS
LMKSMSNDEKATIRGFHLLTTKPVMYIANVAEDGFENNPLLDVVRAIAEEEGAMLVPVCN
KIEAEIAELDDGEEKDMFLEALGLEEPGLNRVIRAGYEMLHLQTYFTAGVEEVRAWTVRV
GATAPQAAGVIHTDFEKGFIRAEVVAYDDFIQFKGESGAKEAGKWRLEGKEYIVKDGDVM
HFRFNV