Protein Info for Psyr_0937 in Pseudomonas syringae pv. syringae B728a

Annotation: mannose-6-phosphate isomerase, type 2 / mannose-1-phosphate guanylyltransferase (GDP)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01479: mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase
  • amino acids 3 to 469 (467 residues), 697.5 bits, E=4.3e-214
• PF00483: NTP_transferase
  • amino acids 4 to 286 (283 residues), 187.3 bits, E=9e-59
• PF12804: NTP_transf_3
  • amino acids 5 to 135 (131 residues), 38 bits, E=4.8e-13
• PF22640: ManC_GMP_beta-helix
  • amino acids 293 to 347 (55 residues), 71.7 bits, 9.1e-24
• PF01050: MannoseP_isomer
  • amino acids 352 to 465 (114 residues), 180 bits, E=3.8e-57
• PF07883: Cupin_2
  • amino acids 384 to 449 (66 residues), 42.1 bits, E=1.5e-14

Best Hits

Swiss-Prot: 58% identical to RFBA_VIBCH: Putative mannose-1-phosphate guanylyltransferase (rfbA) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K00971, mannose-1-phosphate guanylyltransferase [EC: 2.7.7.22] (inferred from 100% identity to psb:Psyr_0937)

MetaCyc: 55% identical to phosphomannose isomerase / GDP-D-mannose pyrophosphorylase (Pseudomonas aeruginosa)
Mannose-6-phosphate isomerase. [EC: 5.3.1.8]; Mannose-1-phosphate guanylyltransferase. [EC: 5.3.1.8, 2.7.7.13]

Predicted SEED Role

No annotation

MetaCyc Pathways

• GDP-mannose biosynthesis (4/4 steps found)
• colanic acid building blocks biosynthesis (9/11 steps found)
• β-1,4-D-mannosyl-N-acetyl-D-glucosamine degradation (2/3 steps found)
• D-mannose degradation I (1/2 steps found)
• D-mannose degradation II (1/2 steps found)
• mannitol degradation II (2/4 steps found)
• mannitol biosynthesis (1/3 steps found)
• 1,5-anhydrofructose degradation (2/5 steps found)
• β-(1,4)-mannan degradation (2/7 steps found)
• superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis (7/14 steps found)

KEGG Metabolic Maps

• Fructose and mannose metabolism
• Nucleotide sugars metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.22, 5.3.1.8

Use Curated BLAST to search for 2.7.7.13 or 2.7.7.22 or 5.3.1.8

Sequence Analysis Tools

See Q4ZXX7 at UniProt or InterPro

Protein Sequence (472 amino acids)

>Psyr_0937 mannose-6-phosphate isomerase, type 2 / mannose-1-phosphate guanylyltransferase (GDP) (Pseudomonas syringae pv. syringae B728a)
MFTPVILAGGSGSRLWPLSRQSFPKQFLALDGQDQGTMFQRTLARLKGLEHAPAVVVSNE
NHRFIVAEQLRVAKMGSRRVILEPLARNTAPAIALAALEATADGTDPILLVLAADHHIHD
EEAFRQAVAVAQVHAEAGRLVTFGITPTHAETGFGYIHCGESIAQGGFAIEAFKEKPSPE
MAAEYLSSGAYLWNSGMFMFRASVFLAELKKHRSDILSACRVALADSDADSYFLHVSAEK
FALCADESVDYAVMEHTDLGLVVPLDAGWNDLGSWAAIWDVGPHDENVNRLEGDVMAIDT
RNCLVQSHHRLVATVGVEDLIVIETKDAVLVANKHNSQQVKDVVKRLQAEERPEFVTHPL
VNRPWGHYDTIDLGERYQVKRISVLPGECLSLQMHYHRAEHWIVVSGTAKVICDDKELIL
SENQSTYIPLGVKHSLANPGKVPLELIEVQSGSYLGEDDIVRFEDRYGRLKK