Protein Info for Psyr_0908 in Pseudomonas syringae pv. syringae B728a
Annotation: FMN-dependent alpha-hydroxy acid dehydrogenase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to LLDD_PSEU2: L-lactate dehydrogenase (lldD) from Pseudomonas syringae pv. syringae (strain B728a)
KEGG orthology group: K00101, L-lactate dehydrogenase (cytochrome) [EC: 1.1.2.3] (inferred from 100% identity to psb:Psyr_0908)MetaCyc: 72% identical to L-lactate dehydrogenase (Escherichia coli K-12 substr. MG1655)
1.1.5.M6 [EC: 1.1.5.M6]; RXN0-7227 [EC: 1.1.5.M6]
Predicted SEED Role
"L-lactate dehydrogenase (EC 1.1.2.3)" in subsystem L-rhamnose utilization or Lactate utilization or Respiratory dehydrogenases 1 (EC 1.1.2.3)
MetaCyc Pathways
- L-lactaldehyde degradation (aerobic) (1/2 steps found)
- methylglyoxal degradation IV (1/3 steps found)
- methylglyoxal degradation V (1/3 steps found)
- superpathway of methylglyoxal degradation (4/8 steps found)
- lactate fermentation to acetate, CO2 and hydrogen (Desulfovibrionales) (3/8 steps found)
- superpathway of fucose and rhamnose degradation (3/12 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 1.1.2.3 or 1.1.5.M6
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q4ZY06 at UniProt or InterPro
Protein Sequence (380 amino acids)
>Psyr_0908 FMN-dependent alpha-hydroxy acid dehydrogenase (Pseudomonas syringae pv. syringae B728a) MIISSASDYRAAAKRKLPRFLFDYIDGGAYAEHTLRANGSDLADISLRQRVLKNVDNVSL ETRLFGESLAMPIILSPVGLSGMYARRGEVQVARAAANKRIPFCLSTVSVCSIEEVASQS DQAIWFQLYVLKDRGFMKNALERAKAAGVTTLVFTVDMPTPGARYRDAHSGMSGPYAAPR RILQAMTKPDWALNVGLLGRPHDLGNISRYLGKATTLEDYVGWLANNFDPSISWKDLEWI REFWQGPMIIKGILDPQDARDALSFGADGIVVSNHGGRQLDGVLSTAKALPPIVQAVGSD LTVLADSGIRSGLDVVRMLALGAKGVLLGRSMAYALGADGQRGVENMLDIFAREMHVAMT LTGVTSIEQIDASILVKAVA