Protein Info for Psyr_0888 in Pseudomonas syringae pv. syringae B728a
Annotation: 3-phosphoshikimate 1-carboxyvinyltransferase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to AROA_PSEU2: 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) from Pseudomonas syringae pv. syringae (strain B728a)
KEGG orthology group: K00800, 3-phosphoshikimate 1-carboxyvinyltransferase [EC: 2.5.1.19] (inferred from 100% identity to psb:Psyr_0888)Predicted SEED Role
"5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19)" in subsystem Chorismate Synthesis or Common Pathway For Synthesis of Aromatic Compounds (DAHP synthase to chorismate) (EC 2.5.1.19)
MetaCyc Pathways
- superpathway of aromatic amino acid biosynthesis (18/18 steps found)
- superpathway of L-tryptophan biosynthesis (13/13 steps found)
- superpathway of L-phenylalanine biosynthesis (10/10 steps found)
- superpathway of L-tyrosine biosynthesis (10/10 steps found)
- chorismate biosynthesis I (7/7 steps found)
- chorismate biosynthesis from 3-dehydroquinate (5/5 steps found)
- superpathway of chorismate metabolism (42/59 steps found)
- chorismate biosynthesis II (archaea) (8/12 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Phenylalanine, tyrosine and tryptophan biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 2.5.1.19
Use Curated BLAST to search for 2.5.1.19
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q4ZY26 at UniProt or InterPro
Protein Sequence (418 amino acids)
>Psyr_0888 3-phosphoshikimate 1-carboxyvinyltransferase (Pseudomonas syringae pv. syringae B728a) MRPQATLTVLPVERPLVGRVSPPGSKSITNRALLLAGLAKGTSRLTGALKSDDTRVMSEA LRLMGVQVDEPDDSTFVVTSSGHWQAPQQALFLGNAGTATRFLTAALANFEGDFVVDGDE YMRKRPIGPLVDALQRMGVEVSAPSGCPPVAIKGKGGLEAGRIEIDGNLSSQYVSALLMA GACGKGPVEVALTGSEIGARGYLDLTLAAMRAFGAEVQAIGDAAWKVSATGYRATDFHIE PDASAATYLWAAQALTEGAIDLGVASNAFTQPDALASQIIASFPNMPAVIDGSQMQDAIP TLAVLAAFNRQPVRFVGIANLRVKECDRISALSNGLCAIAPGLAVEEGDDLIVTANPTLA GTTVDALIDTHSDHRIAMCFALAGLKIAGIRILDPDCVAKTYPGYWDALASLGVSVQR