Protein Info for Psyr_0852 in Pseudomonas syringae pv. syringae B728a

Annotation: pectate lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 415 signal peptide" amino acids 1 to 19 (19 residues), see Phobius details PF22842: Pel9A-like_beta_helix" amino acids 16 to 328 (313 residues), 219.8 bits, see alignment E=8.3e-69 PF07602: DUF1565" amino acids 26 to 67 (42 residues), 21.1 bits, see alignment 2.6e-08 PF13229: Beta_helix" amino acids 147 to 316 (170 residues), 34.5 bits, see alignment E=2.3e-12

Best Hits

KEGG orthology group: None (inferred from 100% identity to psb:Psyr_0852)

Predicted SEED Role

"Pectate lyase L precursor (EC 4.2.2.2)" (EC 4.2.2.2)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.2.2

Use Curated BLAST to search for 4.2.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZY62 at UniProt or InterPro

Protein Sequence (415 amino acids)

>Psyr_0852 pectate lyase (Pseudomonas syringae pv. syringae B728a)
MRTILLTVLLVGAATAHATDYYVAPNGDDHAAGSKGAPLRTIMQAQQAAKAGDTVYFRGG
LYAYTAGINRCATRTDTVNAITLNNSGSENKPIRYWAYPGETPVFDFSAMKDDCRVKGFN
VTGSWLHLKGLEVTGVPQQPENHLNHESWGIWNSGSHNTFEQLNLHHNMGPGLFIQNGGY
NLVLNTDSHHNYDPYTSNGAGQSADGFGAHIKAGHPGNVFRGCRAWANSDDGFDLINAFS
PVTIESSWAWQQGYLPGTRTKLEAGNGNGIKAGGYGGKYVPDGVKHSVRNSVAFDNKSAG
FYANHHTLALDFINNTAFANGVNYNMAGIAPDGSLIPLGNLFNNIAYKGRLTVNTEGLDM
AHNSWTLPTPVTDADFEDVSDTGWDAPRQSDGSLPVLRSFHLRPGSRLAGMGAFH