Protein Info for Psyr_0848 in Pseudomonas syringae pv. syringae B728a

Annotation: ketol-acid reductoisomerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02826: 2-Hacid_dh_C
  • amino acids 13 to 83 (71 residues), 27.8 bits, E=4e-10
• TIGR00465: ketol-acid reductoisomerase
  • amino acids 14 to 326 (313 residues), 430 bits, E=2.3e-133
• PF07991: IlvN
  • amino acids 14 to 177 (164 residues), 250.4 bits, E=1.6e-78
• PF03446: NAD_binding_2
  • amino acids 18 to 103 (86 residues), 24.3 bits, E=7.3e-09
• PF03807: F420_oxidored
  • amino acids 18 to 91 (74 residues), 23.4 bits, E=1.9e-08
• PF01450: IlvC
  • amino acids 183 to 326 (144 residues), 204.2 bits, E=2.7e-64

Best Hits

Swiss-Prot: 100% identical to ILVC_PSE14: Ketol-acid reductoisomerase (NADP(+)) (ilvC) from Pseudomonas savastanoi pv. phaseolicola (strain 1448A / Race 6)

KEGG orthology group: K00053, ketol-acid reductoisomerase [EC: 1.1.1.86] (inferred from 99% identity to pst:PSPTO_0983)

MetaCyc: 70% identical to acetohydroxyacid isomeroreductase (Cupriavidus necator H16)
Ketol-acid reductoisomerase. [EC: 1.1.1.86]; 1.1.1.86 [EC: 1.1.1.86]

Predicted SEED Role

"Ketol-acid reductoisomerase (EC 1.1.1.86)" in subsystem Branched-Chain Amino Acid Biosynthesis or Coenzyme A Biosynthesis (EC 1.1.1.86)

MetaCyc Pathways

• superpathway of branched chain amino acid biosynthesis (17/17 steps found)
• superpathway of L-isoleucine biosynthesis I (13/13 steps found)
• L-isoleucine biosynthesis I (from threonine) (7/7 steps found)
• superpathway of coenzyme A biosynthesis II (plants) (9/10 steps found)
• superpathway of coenzyme A biosynthesis I (bacteria) (8/9 steps found)
• L-valine biosynthesis (4/4 steps found)
• phosphopantothenate biosynthesis I (4/4 steps found)
• pyruvate fermentation to isobutanol (engineered) (4/5 steps found)
• phosphopantothenate biosynthesis III (archaea) (2/4 steps found)
• L-isoleucine biosynthesis III (4/7 steps found)
• superpathway of L-threonine metabolism (11/18 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Pantothenate and CoA biosynthesis
• Valine, leucine and isoleucine biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.86

Sequence Analysis Tools

See Q4ZY66 at UniProt or InterPro

Protein Sequence (338 amino acids)

>Psyr_0848 ketol-acid reductoisomerase (Pseudomonas syringae pv. syringae B728a)
MKVFYDKDCDLSIIQGKKVAIIGYGSQGHAQACNLKDSGVDVTVGLRKGSATVAKAEAHG
LKVTDVASAVAAADLVMILTPDEFQSQLYKNEVEPNLKKGATLAFSHGFAIHYNQVVPRA
DLDVIMIAPKAPGHTVRTEFVKGGGIPDLIAVYQDASGNAKNVALSYASGVGGGRTGIIE
TTFKDETETDLFGEQAVLCGGTVELVKAGFETLVEAGYAPEMAYFECLHELKLIVDLMYE
GGIANMNYSISNNAEYGEYVTGPEVINAESRQAMRNALKRIQDGEYAKMFISEGATGYPS
MTAKRRNNAAHGIEIIGEKLRSMMPWIAANKIVDKDKN