Protein Info for Psyr_0823 in Pseudomonas syringae pv. syringae B728a

Annotation: PTS system D-fructose-specific IIB component (F1P-forming), Frc family / PTS system D-fructose-specific IIC component (F1P-forming), Frc family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 580 transmembrane" amino acids 244 to 270 (27 residues), see Phobius details amino acids 284 to 308 (25 residues), see Phobius details amino acids 319 to 343 (25 residues), see Phobius details amino acids 362 to 383 (22 residues), see Phobius details amino acids 403 to 423 (21 residues), see Phobius details amino acids 442 to 465 (24 residues), see Phobius details amino acids 477 to 497 (21 residues), see Phobius details amino acids 503 to 523 (21 residues), see Phobius details amino acids 544 to 565 (22 residues), see Phobius details PF02302: PTS_IIB" amino acids 5 to 94 (90 residues), 41.4 bits, see alignment E=1.9e-14 amino acids 125 to 214 (90 residues), 76.1 bits, see alignment E=2.9e-25 TIGR00829: PTS system, Fru family, IIB component" amino acids 124 to 207 (84 residues), 112.7 bits, see alignment E=7.7e-37 TIGR01427: PTS system, Fru family, IIC component" amino acids 231 to 566 (336 residues), 475.3 bits, see alignment E=1.3e-146 PF02378: PTS_EIIC" amino acids 247 to 508 (262 residues), 77.1 bits, see alignment E=1.3e-25

Best Hits

Swiss-Prot: 62% identical to PTFBC_ECOLI: PTS system fructose-specific EIIB'BC component (fruA) from Escherichia coli (strain K12)

KEGG orthology group: K02769, PTS system, fructose-specific IIB component [EC: 2.7.1.69] K02770, PTS system, fructose-specific IIC component (inferred from 100% identity to psb:Psyr_0823)

MetaCyc: 62% identical to fructose-specific PTS multiphosphoryl transfer protein FruA (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

No annotation

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69

Use Curated BLAST to search for 2.7.1.69

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZY91 at UniProt or InterPro

Protein Sequence (580 amino acids)

>Psyr_0823 PTS system D-fructose-specific IIB component (F1P-forming), Frc family / PTS system D-fructose-specific IIC component (F1P-forming), Frc family (Pseudomonas syringae pv. syringae B728a)
MKLAIVTACPSGKVSSVLSARLLEAAALRQGWETSVEIIDPNKADQQLSAEDIEAADLVL
VVNTGPVDLSRFVGKRLFQDSPAHALQDVDSFLQRAEREAQVHAASEAAAPQASGGSGGQ
PRIVAITACPTGVAHTFMAAEAIQQAAKRLNYDLQVETQGSVGARNPLSAKAIADADVVL
LAADIEVNTDRFAGKKIYRCGTGIALKQSEATLKKALAEGQVESDEAAAKSPARQEKAGV
YKHLLTGVSFMLPMVVAGGLLIALSFVFGITAFKEPGTLAAALMQIGGEAAFKLMVPLLA
GYIAYSIADRPGLAPGMIGGLLASTLGAGFIGGIVAGFLAGYSAAAINRYARLPASVEAL
KPILIIPLLSSLFTGLVMIYVVGKPVAGMLEALTHFLDSMGTTNAILLGVVLGAMMCVDL
GGPINKASYAFSVGLLASQSYAPMATAMAAGMVPPIGMGIATILARRKFAQSEREAGKAA
FVLGLCFISEGAIPFAAKDPLRVIPASVVGGALTGALSMYFGCKLMAPHGGLFVMLIPNA
INHALLYLLAIIAGSVLTGVIYALIKRPEPADMEVAPAAA