Protein Info for Psyr_0821 in Pseudomonas syringae pv. syringae B728a

Annotation: PTS system D-fructose-specific IIA component (F1P-forming), Frc family / Phosphocarrier protein HPr / phosphoenolpyruvate--protein phosphotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 956 PF00359: PTS_EIIA_2" amino acids 5 to 140 (136 residues), 105.1 bits, see alignment E=7.9e-34 PF00381: PTS-HPr" amino acids 284 to 366 (83 residues), 67 bits, see alignment E=3.1e-22 TIGR01003: phosphocarrier, HPr family" amino acids 285 to 365 (81 residues), 49.4 bits, see alignment E=3.5e-17 PF05524: PEP-utilisers_N" amino acids 407 to 522 (116 residues), 85.5 bits, see alignment E=8.2e-28 TIGR01417: phosphoenolpyruvate-protein phosphotransferase" amino acids 408 to 954 (547 residues), 505.1 bits, see alignment E=2.3e-155 PF00391: PEP-utilizers" amino acids 547 to 619 (73 residues), 74.3 bits, see alignment 1.3e-24 PF02896: PEP-utilizers_C" amino acids 648 to 928 (281 residues), 358.4 bits, see alignment E=6.8e-111

Best Hits

KEGG orthology group: K02768, PTS system, fructose-specific IIA component [EC: 2.7.1.69] K08483, phosphotransferase system, enzyme I, PtsI [EC: 2.7.3.9] K11183, phosphocarrier protein FPr (inferred from 100% identity to psb:Psyr_0821)

Predicted SEED Role

"Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9)" in subsystem Fructose and Mannose Inducible PTS or Fructose utilization or Mannitol Utilization (EC 2.7.3.9)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69, 2.7.3.9

Use Curated BLAST to search for 2.7.1.69 or 2.7.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZY93 at UniProt or InterPro

Protein Sequence (956 amino acids)

>Psyr_0821 PTS system D-fructose-specific IIA component (F1P-forming), Frc family / Phosphocarrier protein HPr / phosphoenolpyruvate--protein phosphotransferase (Pseudomonas syringae pv. syringae B728a)
MLELTLEQISMAQSAVDKTAALKLIADHLVADGLVAEGYLTGLMNREQQGSTFLGQGIAI
PHGTPETRDLVFTTGVRLMQFPEGVDWGDGQMVYLAIGIAAKSDEHLRLLQLLTRALGEE
DLGQALREAKTPEDLLKLLQGAPQELALDAQMISLGVSADDFEELVWRGARLLRKADCVS
NGFAAVLQQVEALSLGDGLWWLHSEQTVKRPGLAFVTPDKPMRYLGQPLTGLFCLASLGE
AHQALLERLCLLLIEGRGHELGHATNSRAVLEALGGELPAEWPTRQIQLANAHGLHARPA
KILAQLAKEFDGEIRMRVLETGEAAVSAKSLSKLLSLGARRGQTLEFIAEPTIADDALPA
LIAAVEQGLGEEVEPLPAVSQTEAAPAPAPAAAKPLSAPPAGSVLQAVSASPGIAMGPAH
VQVLQSFDYPQRGESVAAERERLHKAIGEVRSDIENLIQRSKSKAIREIFITHQEMLEDP
ELTSEVEARLNNDESAAAAWATVIETAAVQQEQLQDALLAERAADLRDVGRRVLAQICGV
ETVAAPDEPYILVMDEVGPSDVARLDPAQVAGILTARGGATAHSAIVARALGIPALVGAG
DEVLLLKPGTVLLLDSQRGRLTVAPDEATLQRAVQDRDAREQRLKAAAAARMEPAVTRDG
HAVEVFANIGDSTGTPAAVEQGAEGVGLLRTELLFMAHSQAPDEATQEAEYRRVLTDLGG
RPLVVRTLDVGGDKPLPYWPIAKEENPFLGVRGIRLTLQRPDVMESQLRALLRSADSGPL
RIMFPMIGTLEEWRQARDMTQRLREEIPVSDLQLGIMIEVPSAALIAPVLAKEVDFFSIG
TNDLTQYTMAIDRGHPTLSAQADGLHPSVLQLIDMTVRAAHANGKWVGVCGELAADPLAV
PILVGLGVDELSVSARSIGEVKACVRELTLSSARELAQNALTAGSAAEVRALVEAV