Protein Info for Psyr_0709 in Pseudomonas syringae pv. syringae B728a

Annotation: FMN adenylyltransferase / riboflavin kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF06574: FAD_syn
  • amino acids 18 to 172 (155 residues), 190.1 bits, E=2.4e-60
• TIGR00083: riboflavin biosynthesis protein RibF
  • amino acids 26 to 310 (285 residues), 307.5 bits, E=5e-96
• PF01687: Flavokinase
  • amino acids 190 to 310 (121 residues), 141.3 bits, E=1.9e-45

Best Hits

Swiss-Prot: 86% identical to RIBF_PSEFL: Bifunctional riboflavin kinase/FMN adenylyltransferase (ribF) from Pseudomonas fluorescens

KEGG orthology group: K11753, riboflavin kinase / FMN adenylyltransferase [EC: 2.7.1.26 2.7.7.2] (inferred from 100% identity to psb:Psyr_0709)

MetaCyc: 53% identical to bifunctional riboflavin kinase / FMN adenylyltransferase (Escherichia coli K-12 substr. MG1655)
FAD synthetase. [EC: 2.7.7.2]; Riboflavin kinase. [EC: 2.7.7.2, 2.7.1.26]

Predicted SEED Role

"Riboflavin kinase (EC 2.7.1.26) / FMN adenylyltransferase (EC 2.7.7.2)" in subsystem Riboflavin, FMN and FAD metabolism (EC 2.7.1.26, EC 2.7.7.2)

MetaCyc Pathways

• adenosylcobalamin biosynthesis II (aerobic) (31/33 steps found)
• flavin biosynthesis I (bacteria and plants) (8/9 steps found)
• flavin salvage (2/2 steps found)
• flavin biosynthesis III (fungi) (7/9 steps found)
• 5,6-dimethylbenzimidazole biosynthesis I (aerobic) (2/3 steps found)
• flavin biosynthesis II (archaea) (5/10 steps found)
• roseoflavin biosynthesis (1/7 steps found)

KEGG Metabolic Maps

• Riboflavin metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.26 or 2.7.7.2

Sequence Analysis Tools

See Q4ZYJ5 at UniProt or InterPro

Protein Sequence (314 amino acids)

>Psyr_0709 FMN adenylyltransferase / riboflavin kinase (Pseudomonas syringae pv. syringae B728a)
MSKKCVMQLVRGLHNLRPEHRGCVATIGNFDGVHRGHQAILARLRERAVELGVPSCVVIF
EPQPREFFAPETAPARLARLRDKLELLSAEGVDRVLCLSFNQRLCKLSAASFVETVLIDG
LGVQHLEVGDDFRFGFDRVGDFDFLQQAGTSYGFTVEAAQTVEIDGIRVSSTKVRKALAA
SDFALAERMLGRPFQIAGRVLHGQKLARQLGTPTANVQLKRRRVPLSGVYLVSTDIDGKT
WPGVANIGVRPTVTGDGSAHLEVHLLDFAGDLYDRRLTVAFHHKLRDEQRFASLEALKTA
INADVAAARAHWHG