Protein Info for Psyr_0689 in Pseudomonas syringae pv. syringae B728a

Annotation: methylmalonate-semialdehyde dehydrogenase (acylating)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 58% identical to MMSA_HUMAN: Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial (ALDH6A1) from Homo sapiens

KEGG orthology group: K00140, methylmalonate-semialdehyde dehydrogenase [EC: 1.2.1.27] (inferred from 100% identity to psb:Psyr_0689)

MetaCyc: 70% identical to methylmalonate-semialdehyde dehydrogenase subunit (Burkholderia cepacia R34)
Methylmalonate-semialdehyde dehydrogenase (acylating). [EC: 1.2.1.27]

Predicted SEED Role

"Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27)" in subsystem Isobutyryl-CoA to Propionyl-CoA Module or Valine degradation (EC 1.2.1.27)

MetaCyc Pathways

myo-inositol degradation I (6/7 steps found)
β-alanine degradation II (2/2 steps found)
L-valine degradation I (6/8 steps found)
β-alanine degradation I (1/2 steps found)
propanoyl-CoA degradation II (3/5 steps found)
myo-, chiro- and scyllo-inositol degradation (6/10 steps found)
2,4-dinitrotoluene degradation (1/7 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.27

Use Curated BLAST to search for 1.2.1.27

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZYL3 at UniProt or InterPro

Protein Sequence (508 amino acids)

>Psyr_0689 methylmalonate-semialdehyde dehydrogenase (acylating) (Pseudomonas syringae pv. syringae B728a)
MNASLNHTTTSIARVKLLINGEWTDSQSSEWQDIVNPATQEILAQVPFATASEVDAAVAA
AHAAYRTWRETPIGVRMRIMLKLQALIREHSKRIAAVLSAEQGKTLADAEGDIFRGLEVV
EHACSIGTLQMGEFAENVASGVDTYTLRQPIGVCAGITPFNFPAMIPLWMFPMAIACGNT
FVLKPSEQDPLSTLLLVELAIEAGVPPGVLNVVHGGKEVVDAICTHKDIKAVSFVGSTAV
GTHVYDLAGRHGKRVQAMMGAKNHAVVLPDANREQTLNALVGAGFGAAGQRCMATSVVVL
VGASKQWLSDLKARAQKLKVNAGQEPGTDIGPVISRRAKQRILDLIESGVREGATLELDG
REVSVPGYEGGNFVGPTLFSGVTTDMQIYTQEIFGPVLVVLEVDTLDEAIALVNANPFGN
GTGLFTQSGAAARKFQSEIDVGQVGINIPIPVPAPFFSFTGSRGSKLGDLGPYGKQVVQF
YTQTKTVTSRWFDDDSVNDGVNTTINLR