Protein Info for Psyr_0682 in Pseudomonas syringae pv. syringae B728a

Annotation: DNA helicase/exodeoxyribonuclease V, alpha subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 697 TIGR01447: exodeoxyribonuclease V, alpha subunit" amino acids 34 to 695 (662 residues), 668 bits, see alignment E=6.6e-205 PF21185: RecD_N" amino acids 36 to 157 (122 residues), 39.7 bits, see alignment E=1.9e-13 PF13245: AAA_19" amino acids 208 to 360 (153 residues), 109.6 bits, see alignment E=5.3e-35 PF13604: AAA_30" amino acids 213 to 374 (162 residues), 91.9 bits, see alignment E=1.8e-29 PF13086: AAA_11" amino acids 216 to 281 (66 residues), 29.3 bits, see alignment E=3.1e-10 PF13401: AAA_22" amino acids 217 to 352 (136 residues), 35.7 bits, see alignment E=4.2e-12 PF05127: Helicase_RecD" amino acids 222 to 340 (119 residues), 39.1 bits, see alignment E=2.9e-13 PF13538: UvrD_C_2" amino acids 625 to 671 (47 residues), 45.6 bits, see alignment 2e-15

Best Hits

KEGG orthology group: K03581, exodeoxyribonuclease V alpha subunit [EC: 3.1.11.5] (inferred from 100% identity to psb:Psyr_0682)

Predicted SEED Role

"Exodeoxyribonuclease V alpha chain (EC 3.1.11.5)" in subsystem DNA-replication (EC 3.1.11.5)

Isozymes

Compare fitness of predicted isozymes for: 3.1.11.5

Use Curated BLAST to search for 3.1.11.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZYM0 at UniProt or InterPro

Protein Sequence (697 amino acids)

>Psyr_0682 DNA helicase/exodeoxyribonuclease V, alpha subunit (Pseudomonas syringae pv. syringae B728a)
MSRSFDDLLPTALDDISLAELSPLTRVSDLLILLERWVERGWLRALDKAFVAFLSDLDPQ
ADPLVLVAAALTSHQLGHGHVCLDLYETLKEPDFALSLPPEGDQQSAPMLLPSQLLAALD
GAAWCQALAASMLVAEVGDLSAEARQKPLVLAERRLYLRRYWTYERRIAAALRQRLAQRE
TPPEGLPQRLDALFGPADSSPQAVIDWQKLACALATRKGFSIITGGPGTGKTTTVVRLLA
LLQAPAVQSGQPLRIRLAAPTGKAAARLTESISQQVQSLDVSDDVRQKIPSEVTTVHRLL
GSRPGTRHFRHHAGNLLPLDVLVVDEASMIDLEMMANLLYALPAHARMVLLGDKDQLASV
EAGAVLGDLCRDAEEGFYSPDTQAWLEAVSGEDLGKGGLRQGDAQQHPLAQQVVMLRHSR
RFGQGSGIGQLARLVNQQQDQQARALLSAGSHDDLFALALKGEQDLKFERLVLDGLGRGE
QGPQGYRHYLNVLAAERPADDSALDDACWTRWARSVLSAFDAFQLLCAVRKGPWGVEGLN
QRITHALFSAKLIESEQQWYEGRPVLMTHNDYGLGLMNGDIGITLRLPERDGEGKQVLRV
AFPRNDGSEGVRFVLPSRLNEVETVYAMTVHKSQGSEFAHTALILPEALNPVLTKELIYT
GITRAKHWFSLIEPRQGIFEEALRRKVKRLSGLMLGL