Protein Info for Psyr_0681 in Pseudomonas syringae pv. syringae B728a

Annotation: DNA helicase/exodeoxyribonuclease V, beta subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1230 TIGR00609: exodeoxyribonuclease V, beta subunit" amino acids 10 to 1216 (1207 residues), 1089 bits, see alignment E=0 PF00580: UvrD-helicase" amino acids 14 to 445 (432 residues), 165.4 bits, see alignment E=5.4e-52 PF13245: AAA_19" amino acids 19 to 433 (415 residues), 42.2 bits, see alignment E=2.2e-14 PF13361: UvrD_C" amino acids 701 to 839 (139 residues), 43.4 bits, see alignment E=7.7e-15 PF12705: PDDEXK_1" amino acids 987 to 1191 (205 residues), 24.5 bits, see alignment E=6.4e-09

Best Hits

KEGG orthology group: K03582, exodeoxyribonuclease V beta subunit [EC: 3.1.11.5] (inferred from 76% identity to pba:PSEBR_a683)

Predicted SEED Role

"Exodeoxyribonuclease V beta chain (EC 3.1.11.5)" in subsystem DNA-replication (EC 3.1.11.5)

Isozymes

Compare fitness of predicted isozymes for: 3.1.11.5

Use Curated BLAST to search for 3.1.11.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZYM1 at UniProt or InterPro

Protein Sequence (1230 amino acids)

>Psyr_0681 DNA helicase/exodeoxyribonuclease V, beta subunit (Pseudomonas syringae pv. syringae B728a)
MSEQSLPLALRFPLRGSQLIEASAGTGKTFTISALYLRLVLGHGTEQCGFGRELLPPQIL
VVTFTDAATKELRDRIRTRLAEAARFFRDEIPSPDGLIAELREQFEASLWPACASRLDIA
AQWMDEAAVSTIHSWCQRMLREHAFDSGSLFTQTLETDHSDLLGEVLRDYWRRFCYPMSG
DALTWVRENLSGPAALLPRVRGLFGRDRTPLTQEPAELIAASLQERRAALAALKAPWAVW
SDELYALCQDAVARKAVDGRKMQERYFKPWFEKLAAWAADDTQEALDLGTGFTRLTPDGI
AEAWKGDPPAHPAFEAMAQLKAALDGLPKPDAAVLQHAAQWVSVRFEEEKRRRAEMGFDD
MLLRLDAALHGAGGERLATLIREQFPVALIDEFQDTDPVQYRIFDSIYRLEANDEQTGLF
LIGDPKQAIYAFRGADIYTYLRARQATDGRWHTLDTNYRSSHAMVESVNHVFTRAEQREQ
GRGAFLFRDEHGNQVPFSDALAQGRKETLHVDGAPLTALTVWHLQSDQPVSGVVYRQQLA
ASCASEIVRLLNAGQQGSAGFVTPDSTLRGLRPADIAILVRDGKEAQAVRQQLTARGVRS
VYLSDKDSVFAAQEAHDLLSWLKACAEPDSERTLRAALACITLDLPLAELERLNQDELAW
ERRVMQFRGYRAIWRSQGVLPMLRRLLHDFALPQTLMTRNDGERVLTNLLHLSELLQQAA
AELDGEQALIRHLGDHLALSGQAGEEQILRLESDEQLVKVVTIHKSKGLQYPLVFLPFIC
SSKPVDGSRLPLQFHDADGKSQISLQPTEALIQQADDERLAEDLRLLYVALTRSEHACWL
GVADLKRGNANTSMLHRSALGYLLGGGAALAESAELNIWLRDLAQGCDAIALQPVPEPTD
ERFSAPRNAAALVKLLVPKRRAAENWWIASYSALRIGDTVTAGADESPDSPQAQKLFDDE
RLDPEAPRDILTSGGDIHRFPRGPNPGTFLHGLLEWAGNEGFANTAHAPDKLQDAVARRC
NRRGWAGWINTLSDWLQHLLNMPLRLSPDAAPVALGELDQPNQYRVEMEFWFASNNVNVA
QMDALVRRFTHDNAPRAATETVSLNGMFKGFIDLTFEHEGRYYVADYKSNWLGSDDSAYT
PLAMETAILDNRYDLQYVLYLLALHRQLKTRMADYDYDQHMGGALYLFLRGSRSASQGAY
FARPPRELIESLDLLFQGKPIAHDLPGALA