Protein Info for Psyr_0680 in Pseudomonas syringae pv. syringae B728a

Annotation: DNA helicase/exodeoxyribonuclease V, gamma subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1157 signal peptide" amino acids 1 to 17 (17 residues), see Phobius details PF04257: Exonuc_V_gamma" amino acids 11 to 366 (356 residues), 329.4 bits, see alignment E=2.3e-102 TIGR01450: exodeoxyribonuclease V, gamma subunit" amino acids 11 to 1100 (1090 residues), 1211.2 bits, see alignment E=0 PF17946: RecC_C" amino acids 847 to 1084 (238 residues), 168.4 bits, see alignment E=1.9e-53

Best Hits

KEGG orthology group: K03583, exodeoxyribonuclease V gamma subunit [EC: 3.1.11.5] (inferred from 77% identity to pba:PSEBR_a682)

Predicted SEED Role

"Exodeoxyribonuclease V gamma chain (EC 3.1.11.5)" in subsystem DNA-replication (EC 3.1.11.5)

Isozymes

Compare fitness of predicted isozymes for: 3.1.11.5

Use Curated BLAST to search for 3.1.11.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZYM2 at UniProt or InterPro

Protein Sequence (1157 amino acids)

>Psyr_0680 DNA helicase/exodeoxyribonuclease V, gamma subunit (Pseudomonas syringae pv. syringae B728a)
MSVTTSLSAGFMVVQGNRPDELRSLVVSWMRRYPLAPLENEIALVQSNGIAQWLKLALAE
DAQDEDNGGCGIAAAIDVQLPGSFMWTLYRMVLGADEIPQTSLLDKTPLTWRLMRLLPSL
INQPHFEPLQRFLTDDTDLRKRYQLSERLSDLFDQYQVYRADWLQDWAAGRHQLRDVRGQ
SRPLSAANCWQAELWRALLDDVGAEGMSQSRAGVHQRFIERIGNMTEAPPGLPARVIVFG
ISSLPAQALEALAGLARFSQVLLCVHNPCRHHWTDIVADKDLLRHQYKRQARKSGMPMVL
DPQALHQHAHPLLAAWGKQGRDYINLLDSHDDPRSYRSSFKDERIDLFSESEPTNLLNQL
QDDILELRPLDETRELWPAVNPSEDRSIRFHVAHSAQREVEVLHDQLLARFSKDPGLRPR
DVIVMVPDIDSYAPHIRAVFGQIEREDRRFIPFTLADQGQRGREPLLIAVEHLLKLPDSR
FPVSEILDLLDVPALRARFRIQERDLPTLHRWIEGAGIRWGLNAQQRAGLGLPDALEQNS
WHFGLRRMLLGYAVGAGAAYDGIEPYDEIGGLDAALIGPLVALIDALQVAHTELSTPTTP
EAWGARLQSLLQLFFLADSEHDDYLLAQLETLRENWLETCATVRLTDELPLTVVREAWLA
GLDQGRLSQRFLAGSVNFCTLMPMRAIPFKVVCLLGMNDGDYPRAQPPLDFDLMGSDYRP
GDRSRREDDRYLLLEALLSARDQLYVSWVGHSIRDNSERPASVLIGQLRDHLASGWKLAG
ETDPDGKHDDQRLLKALTVNHPLQPFSANYFHAGTGYFSFAREWRLLHETDLQVPVPQAL
PAHEQEEPLSIAQLQDFLRNPVKHFFSQRLKIYFEVAEAPLADEEPFVLDALERYGLSES
LLSAAMVSPDDMDAALKTQALKLQASGLLPLAGFGTLMQSELIEPLPDVLRRYHDLLTLW
PETLSSALPIGFSHAGVSIDGWLGGLHRNARGELLLVTAIPNSIGSKKTRKWHRLIRPWV
NHLVACACDLPLSTALVASDETLMLEPLDKASAVTTLNHLLTAWLHGMQEPLPVAVKTAF
AWLGQPADKAEAAARKAYEGDGQTTDGERRESTALARQFPDFDALIDSEEFAGWCETLYK
PIYDAPWQSLSGGEGGA