Protein Info for Psyr_0657 in Pseudomonas syringae pv. syringae B728a

Annotation: Drug resistance transporter Bcr/CflA subfamily

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 398 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details transmembrane" amino acids 43 to 61 (19 residues), see Phobius details amino acids 71 to 90 (20 residues), see Phobius details amino acids 96 to 117 (22 residues), see Phobius details amino acids 129 to 151 (23 residues), see Phobius details amino acids 159 to 179 (21 residues), see Phobius details amino acids 208 to 232 (25 residues), see Phobius details amino acids 244 to 263 (20 residues), see Phobius details amino acids 274 to 294 (21 residues), see Phobius details amino acids 300 to 322 (23 residues), see Phobius details amino acids 337 to 358 (22 residues), see Phobius details amino acids 364 to 383 (20 residues), see Phobius details TIGR00710: drug resistance transporter, Bcr/CflA subfamily" amino acids 3 to 378 (376 residues), 309 bits, see alignment E=3.1e-96 PF07690: MFS_1" amino acids 16 to 351 (336 residues), 135.9 bits, see alignment E=1.7e-43 PF00083: Sugar_tr" amino acids 38 to 182 (145 residues), 36.8 bits, see alignment E=2.3e-13

Best Hits

Swiss-Prot: 32% identical to BCR_ECOLI: Bicyclomycin resistance protein (bcr) from Escherichia coli (strain K12)

KEGG orthology group: K07552, MFS transporter, DHA1 family, bicyclomycin/chloramphenicol resistance protein (inferred from 100% identity to psb:Psyr_0657)

MetaCyc: 32% identical to multidrug efflux pump Bcr (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-340; TRANS-RXN-44

Predicted SEED Role

"Multidrug resistance transporter, Bcr/CflA family" in subsystem Copper homeostasis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZYP5 at UniProt or InterPro

Protein Sequence (398 amino acids)

>Psyr_0657 Drug resistance transporter Bcr/CflA subfamily (Pseudomonas syringae pv. syringae B728a)
MNLRTILILGSLSAFGPLAIDFYLPGFPAMASYFGTDEKHVQLTLAAYFLGLSLGQLAYG
PVADRFGRRIPLLVGVTLFMLASVACAFAPSLEWLIAARFVQALGGCAGMVLSRAIVSDK
CNAVESAKVFSQLMLVMGLAPILAPMLGGVLVSTFGWQSIFVSLALFSGACLAAVALGLP
ESMPATTPRQPLSGALGQYLNLFKDGVFIGHALTGGIAMAGMFAYVAGSPFVFIKLYGVP
AEHYGWLFGMNAAGFILVAQINARILRKTGPAFLLSRTVWIYLAAGLVLLAISGLRTEAL
WPLLVPLFICVASLGCIIPNASACAMNGQWARAGSASALLGCLQFSVAAVAASLVGVLHD
GTATPMALVISLCGVLTVGLSVLTRRAQARRDASLQTA