Protein Info for Psyr_0554 in Pseudomonas syringae pv. syringae B728a

Annotation: DNA topoisomerase IV subunit A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 751 TIGR01062: DNA topoisomerase IV, A subunit" amino acids 12 to 745 (734 residues), 1120 bits, see alignment E=0 PF00521: DNA_topoisoIV" amino acids 36 to 472 (437 residues), 477.7 bits, see alignment E=3.6e-147 PF03989: DNA_gyraseA_C" amino acids 602 to 639 (38 residues), 15.6 bits, see alignment 9.1e-07 amino acids 650 to 687 (38 residues), 18.3 bits, see alignment 1.2e-07

Best Hits

Swiss-Prot: 87% identical to PARC_PSEAE: DNA topoisomerase 4 subunit A (parC) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K02621, topoisomerase IV subunit A [EC: 5.99.1.-] (inferred from 100% identity to psb:Psyr_0554)

Predicted SEED Role

"Topoisomerase IV subunit A (EC 5.99.1.-)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.-)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.-

Use Curated BLAST to search for 5.99.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZYZ8 at UniProt or InterPro

Protein Sequence (751 amino acids)

>Psyr_0554 DNA topoisomerase IV subunit A (Pseudomonas syringae pv. syringae B728a)
MSDSLDLSLDGVERRSLADFTEQAYLNYSMYVIMDRALPHIGDGLKPVQRRIIYAMSELG
LGADAKHKKSARTVGDVLGKFHPHGDSACYEAMVLMAQPFSYRYTLVDGQGNWGAPDDPK
SFAAMRYTEARLSRYSEVLLSELGQGTADWVPNFDGTLDEPAVLPARLPNILLNGTTGIA
VGMATDVPPHNLREVASACVRLLDDPKATVPELCEHILGPDYPTEAEIITPRADLLKIYE
TGRGSVRMRAVYRIEDGDIIVTALPHQVSGAKVLEQIAAQMQAKKLPMVADLRDESDHEN
PCRIVIIPRSNRVELDELMQHLFATTELESSYRVNINIIGLDGKPQLKNLKTLLGEWLTF
RIGTVRRRLQFRLDKVEHRLHLLDGLLTAYLNLDEVIHIIRTAEHPKAELIARFDLSEIQ
ADYILDTRLRQLARLEEMKLRSEQDALRKEQAKLLALLSSESKLRKLVRAELIADAETYG
DERRSPIVARAEAKALSENELMPTEPVTVVLSEKGWVRCAKGHDIDATGLSYKAGDGFKT
SSIGRSNQFAVFIDSTGRSYSVAAHTLPSARGQGEPLTGKLQPPPGATFECVLLPEDDAL
YVIASDAGYGFVVKGEDLQAKNKAGKALLSLPAGAKVILPRPVPDREQNWLAAVTTEGRL
LVFKISDLPQLGKGKGNKIIGIPGERVASREEYVTDLAVIGQGATLVLQAGKRTLSLKPE
DLEHYKGERGRRGNKLPRGFQRVDALLVENS