Protein Info for Psyr_0519 in Pseudomonas syringae pv. syringae B728a

Annotation: Glutamate-ammonia-ligase adenylyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 985 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details PF03710: GlnE" amino acids 51 to 296 (246 residues), 301 bits, see alignment E=6.7e-94 amino acids 580 to 833 (254 residues), 310 bits, see alignment E=1.2e-96 PF08335: GlnD_UR_UTase" amino acids 317 to 455 (139 residues), 109 bits, see alignment E=2e-35 amino acids 875 to 953 (79 residues), 35.6 bits, see alignment E=9.3e-13

Best Hits

Swiss-Prot: 93% identical to GLNE_PSESM: Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme (glnE) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: K00982, glutamate-ammonia-ligase adenylyltransferase [EC: 2.7.7.42] (inferred from 100% identity to psb:Psyr_0519)

Predicted SEED Role

"Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42)" in subsystem Ammonia assimilation (EC 2.7.7.42)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.42

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZZ33 at UniProt or InterPro

Protein Sequence (985 amino acids)

>Psyr_0519 Glutamate-ammonia-ligase adenylyltransferase (Pseudomonas syringae pv. syringae B728a)
MSLPLLAEFPAILLPSISRARQMFQAALTALSEDALASFQAWPEARQAAFDRVCAASDFV
TEQVCRDPQMLLQLADSGELERSFSAGQLRGQLADALSSVASEDELGRNLRRQRARQQVR
IIWRDLSRQANLIETCRDLSDMADASIDLAYQWLYARHCQQFGTPTGRHSGKPQHMVILG
MGKLGAVELNLSSDIDLIFGYPEGGETVGTKRALDNQEFFIRLGQRLIKALDPVTVDGFV
FRVDMRLRPYGSSGALVLSFNALEQYYQDQGRDWERYAMIKARVVGGDQAAGAELLAMLR
PFVYRRYLDFSAIEALRTMKQLIQQEVKRKGMAENIKLGAGGIREVEFIAQAFQLIHGGR
DLSLQQRPLFKVLKTLEGQGYLPSAVTEELREGYEFLRYTEHAIQAIADRQTQMLPDNKQ
DQARVALIMGFADWAAFHERLMYWRGRVAWHFRQVIADPDADPDDEQDEDGEVVVGGEWL
PLWEESQDEEAAGRQLLQAGFVDAERALKNLANLRSSPNLRSMQRLSRERLDAFIPRLLA
QAVEHEKPDLVLERVLPLVEAVARRSAYLVLLTENPDALRQLLTLCAASPWIAEQIARFP
LLLDELLNEGRLFNPPLAPELAAELRERLIRIPEDDLEQQMEALRHFKLAHSLRVAASEI
SGSLPLMKVSDYLTWLAEAILDQVLALAWRYSVARHGTPLRPDGSLCDPGFVIVGYGKVG
GIELGHGSDLDLVFIHDGDLEAETDGAKPIDTAQFFTRLGQRVIHLLTTQTNSGQLYEVD
MRLRPSGASGLLVSSLGAFARYQANEAWTWEHQALVRARVMTGSRDVGEQFEKVRADVLG
RERDLHKLRAEVSEMRAKMRDNLGTRVTAAGRAANAFEAAVPFDLKQDAGGIVDIEFMVQ
YAALAWSREHPALLQYTDNIRILEGLEEAGLLPDTDAGLLREAYKAYRSAAHRQALQKQA
GVVSGDQFHAQRREVMRIWTQMGLS