Protein Info for Psyr_0490 in Pseudomonas syringae pv. syringae B728a

Annotation: CheW-like protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 179 PF01584: CheW" amino acids 38 to 172 (135 residues), 101.6 bits, see alignment E=1.4e-33

Best Hits

Swiss-Prot: 61% identical to PILI_PSEAE: Protein PilI (pilI) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K02659, twitching motility protein PilI (inferred from 100% identity to psb:Psyr_0490)

Predicted SEED Role

"type IV pili signal transduction protein PilI"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZZ62 at UniProt or InterPro

Protein Sequence (179 amino acids)

>Psyr_0490 CheW-like protein (Pseudomonas syringae pv. syringae B728a)
MAEPRTAFELLLDMDRRCRSLAAGLPLQEAHQQDWVGIGFRMGEQRFVAPIGEIAEILHE
PHHAVMPGVKFWVAGIANLRGRLLPLMDLCGFFGHELVPVRKQRRVLVVEHHDVFAGLLV
DEVFGMQRFSQLSLIPQTPQEIDQRMVPFLRGQFIREQAWLIFSPWALVQSADFMDLAS