Protein Info for Psyr_0374 in Pseudomonas syringae pv. syringae B728a

Annotation: Phosphorylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 816 TIGR02093: glycogen/starch/alpha-glucan phosphorylases" amino acids 12 to 813 (802 residues), 1103.4 bits, see alignment E=0 PF00343: Phosphorylase" amino acids 100 to 813 (714 residues), 1033.3 bits, see alignment E=0

Best Hits

Swiss-Prot: 50% identical to PHSG_SYNY3: Glycogen phosphorylase (glgP) from Synechocystis sp. (strain PCC 6803 / Kazusa)

KEGG orthology group: K00688, starch phosphorylase [EC: 2.4.1.1] (inferred from 100% identity to psb:Psyr_0374)

Predicted SEED Role

"Glycogen phosphorylase (EC 2.4.1.1)" in subsystem Glycogen metabolism or Maltose and Maltodextrin Utilization (EC 2.4.1.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZZH8 at UniProt or InterPro

Protein Sequence (816 amino acids)

>Psyr_0374 Phosphorylase (Pseudomonas syringae pv. syringae B728a)
MSQEPLVRDADVAAFRAAVLAKLTYSVGKDPDHAFEHDWFEAVALAARDHMVEHWMDHTR
QIYRKVQKRVYYLSLEFLIGRLLYDSLSNLGLLEVAREALTELGVDIERIRLLEPDAALG
NGGLGRLAACFMESMSTLGIAGHGYGIRYEHGLFRQGIVDGWQQEQTENWLDFGNPWEFE
RPEVVYSIGFGGSVDTVPTEAGDSRQVWRPGETVRAIAYDTPVVGWRGKSVNTLRLWRAR
AVEDLHLERFNAGDHFGAVAEVVRAESISRVLYPNDATEAGQELRLRQEYFFVSASLQDL
LRRHLNQHATLTDLSEHAAIQMNDTHPSIAVAELMRQLIDNHNIPWDTAWKITVGTLGYT
NHTLLPEALETWSVGLMERMLPRHMQIIYLINAQHIDTLRAKGVDDVNVLRAVSLIEEDN
GRRVRMGNLAFLGSHSVNGVSALHTQLMRKTVFAELHKIYPERINNKTNGITFRRWLFQA
NPKLTEMLVESLGEDVLDNAETRLKELEPFAEKSSFRKQMADQRLHSKRALAAIIHERLG
IAVNPAAMFDVQVKRIHEYKRQLLNLFHTVALYQAIRAEPGTDWVPRVKIFAGKAAASYH
SAKLIIKLTNDIARTVNNDPTVRGLLKVVFMPNYNVSLAESIIPAADLSEQISTAGLEAS
GTSNMKFGLNGALTIGTLDGANVEMSEQVGLEHMFIFGMSSQQVEARKQAGEFSAHDDVA
ASGRLNDVLQAIRGGVFSPDDPNRYVGLIDQLLAYDRFLVCADFDSYWAAQAKVEERWHD
SKEWWRSAVLNTARMGWFSSDRTIREYAGDIWKALE