Protein Info for Psyr_0310 in Pseudomonas syringae pv. syringae B728a

Annotation: HNH endonuclease:S-type Pyocin

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 656 PF06958: Pyocin_S" amino acids 385 to 517 (133 residues), 120.7 bits, see alignment E=7e-39 PF21431: Col-Pyo_DNase" amino acids 527 to 650 (124 residues), 178.2 bits, see alignment E=7e-57

Best Hits

KEGG orthology group: None (inferred from 100% identity to psb:Psyr_0310)

Predicted SEED Role

"pyocin/colicin protein, putative"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZZN9 at UniProt or InterPro

Protein Sequence (656 amino acids)

>Psyr_0310 HNH endonuclease:S-type Pyocin (Pseudomonas syringae pv. syringae B728a)
MADEQEKHIYVKYWPIPDLGSADRAAAEAAANKASGGGNSGWSIGIGGGGGGPSTTGLGP
GRGRRRGITNKQLRRLLRQQEAEAHALAQRQAAEHAAHLARLEAEAHARAMAEAAERERV
ETQVRAQAEKDQAHRQALETLTASYPSLQSGLAQNYLLASASLPNDLQKEIASGHDLNAD
PPRDEQALELILQKKIRVNYLLAIKQPLLEERRAQALSLTGQELDHATQKDHLNYLVYYS
QGDPPRVQQAHEAWIQALSQTYEAKLLAESVTLLNEQSAALSMRHAELSLANKPASQDAR
QAAGIDKLWSVIAPASTTTAATGIRTVATNIAKDQLIRIATRTLGSNLVTLLAMYPQPLG
DAELPPAVIATPLSQLNLPPHIDLHYLASVKGTLDVPHRLTSDEAGTSGAARWVATDGVE
VGTKVRVRTFTYNAQNNSYEFIRDGESTPALIWTPIARPADSSTSSPAGPPALPVDPGNV
VTPFVPELEAYPAIDRDDPDDYILISPIDSGLPNSYLLFKDPRSIPGVASGYGEAVNGVW
LGDKTRAEGASIPAHIADQLRGRRFGNFDSLRKATWIAVANDPELVKQFTQHNLEIMRDG
GAPYPRLVDQAGGRTKFEIHHKKHIANGGAVYDIDNLVIMTPRQHIDHHRSHENDL