Protein Info for Psyr_0293 in Pseudomonas syringae pv. syringae B728a

Annotation: Polyphosphate kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 736 transmembrane" amino acids 464 to 480 (17 residues), see Phobius details TIGR03705: polyphosphate kinase 1" amino acids 53 to 726 (674 residues), 940.4 bits, see alignment E=3.1e-287 PF13089: PP_kinase_N" amino acids 53 to 158 (106 residues), 123.3 bits, see alignment E=1.3e-39 PF02503: PP_kinase" amino acids 169 to 350 (182 residues), 205.2 bits, see alignment E=1.8e-64 PF17941: PP_kinase_C_1" amino acids 377 to 540 (164 residues), 231.9 bits, see alignment E=8.2e-73 PF13090: PP_kinase_C" amino acids 549 to 718 (170 residues), 220.5 bits, see alignment E=2.6e-69

Best Hits

Swiss-Prot: 99% identical to PPK1_PSESM: Polyphosphate kinase (ppk) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: K00937, polyphosphate kinase [EC: 2.7.4.1] (inferred from 100% identity to psb:Psyr_0293)

Predicted SEED Role

"Polyphosphate kinase (EC 2.7.4.1)" in subsystem High affinity phosphate transporter and control of PHO regulon or Phosphate metabolism or Purine conversions (EC 2.7.4.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.4.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZZQ6 at UniProt or InterPro

Protein Sequence (736 amino acids)

>Psyr_0293 Polyphosphate kinase (Pseudomonas syringae pv. syringae B728a)
MNTEALIEAAVQVDVPEATPVVEPDIEVIPAIEAPAAVVPAIVAPNLDDSSLYIHRELSQ
LQFNIRVLEQALDESYPLLERLKFLLIFSSNLDEFFEIRVAGLKKQITFAREQAGADGLQ
PHQALARISELVHGHVDRQYAILNDILLPELEKHQVRFIRRRHWTAKLKAWVRRYFRDEI
APIITPIGLDPTHPFPLLVNKSLNFIVELEGIDAFGRDSGLAIIPAPRLLPRVIKVPEEV
CGPGDNFVFLSSMIHAHADDLFQGMKVKGCYQFRLTRNADLALDSEDVEDLARALRGELF
SRRYGDAVRLEVADTCPKHLSDYLLKQFNLHESELYQVNGPVNLTRLFSITGLDSHPELQ
YPPFTPAIPKLLQNSENVFSVVSKQDILLLHPFESFTPVVDLLRQAAKDPHVLAVRQTLY
RSGANSEIVDALVDAARNGKEVTAVIELRARFDEESNLQLASRLQAAGAVVIYGVVGFKT
HAKMMLILRREAGEIVRYAHLGTGNYHAGNARLYTDYSLLTSDDALCEDVGKLFSQLIGM
GKTLRMKKLLHAPFTLKKGMLDMIARETQFALDGKPAHIIAKFNSLTDPKIIRALYKASQ
SGVRIDLVVRGMCCLRPGIAGVSHNIHVRSIIGRFLEHTRVFYFLNGGDEQMFLSSADWM
ERNLDKRVETCFPVEGKKLILRVKKELESYLTDNTHSWLLQSDGRYVRSTPTGNQNPRSA
QATLLERLSNPVLSVR