Protein Info for Psyr_0264 in Pseudomonas syringae pv. syringae B728a

Annotation: Alginate biosynthesis sensor protein KinB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 595 signal peptide" amino acids 1 to 40 (40 residues), see Phobius details transmembrane" amino acids 168 to 190 (23 residues), see Phobius details PF16767: KinB_sensor" amino acids 39 to 160 (122 residues), 141.8 bits, see alignment E=6.2e-45 PF00672: HAMP" amino acids 193 to 244 (52 residues), 42.9 bits, see alignment 2e-14 TIGR00229: PAS domain S-box protein" amino acids 257 to 373 (117 residues), 38.9 bits, see alignment E=4.2e-14 PF13188: PAS_8" amino acids 258 to 311 (54 residues), 29.4 bits, see alignment 2.3e-10 PF00989: PAS" amino acids 259 to 366 (108 residues), 32 bits, see alignment E=4.2e-11 PF08448: PAS_4" amino acids 264 to 371 (108 residues), 47.5 bits, see alignment E=7.8e-16 PF00512: HisKA" amino acids 376 to 443 (68 residues), 54.5 bits, see alignment E=4e-18 PF02518: HATPase_c" amino acids 490 to 595 (106 residues), 99.8 bits, see alignment E=5.1e-32

Best Hits

KEGG orthology group: K11383, two-component system, NtrC family, sensor histidine kinase KinB [EC: 2.7.13.3] (inferred from 100% identity to psb:Psyr_0264)

Predicted SEED Role

"Sensory box histidine kinase"

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZZT5 at UniProt or InterPro

Protein Sequence (595 amino acids)

>Psyr_0264 Alginate biosynthesis sensor protein KinB (Pseudomonas syringae pv. syringae B728a)
MKLAMKLRTRLFLSISALITVALLGLLLGLVSVMQMARTQESLIQHNFAILDLGLKLRQN
MGDQLVLMTGSSRNEPELQRMQKQFEELLEEGSAQDTQQNVRNGFEKTRGDYQRFIATWQ
QYGNDPRGIRGNSQLSESFDTLRNGLMDVHRKALENISNAEINSRDRALWIAGLLGLVGL
AVLCIGFVTAHGIARRFGAPIEALAKAADRIGEGDYEVTLPISSAAEMNLLTRRFGIMAE
ALRQHQATNVDELLAGQQRLQAVLDSIDDGLLMIDRQGHLEHLNPVAQRQLGWDESRLGH
SLGEALGRPELDEQLHLVLRGGTLERAPEDLAIEIDGESRLLTYSMTPVSHTKGHILGAV
MVLHDVTEQRAFERVRSEFVLRASHELRTPVTGMHMAFGLLQERLHFAPESREADLLNTV
TEEMQRLMQLINDLLNFSRYQNGLQKLKLAPCSIETLLQEAKARFEDQAQEQDIVLMLDM
QEPMPRLHADQSQLERVLDNLLDNALRHTPPNGLIRLQARRHGERAIISVEDNGEGIAYG
QQGRIFEPFVQVGRKKGGAGLGLALCKEIVQLHGGRMGVYSRPGQGTQFYMALPL