Protein Info for Psyr_0209 in Pseudomonas syringae pv. syringae B728a

Annotation: RelA/SpoT protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 707 TIGR00691: RelA/SpoT family protein" amino acids 32 to 703 (672 residues), 795.5 bits, see alignment E=2.1e-243 PF13328: HD_4" amino acids 32 to 181 (150 residues), 173.5 bits, see alignment E=8.2e-55 PF01966: HD" amino acids 51 to 150 (100 residues), 43.9 bits, see alignment E=8.4e-15 PF04607: RelA_SpoT" amino acids 241 to 350 (110 residues), 144.9 bits, see alignment E=3.5e-46 PF02824: TGS" amino acids 395 to 453 (59 residues), 68.8 bits, see alignment 1e-22 PF19296: RelA_AH_RIS" amino acids 466 to 549 (84 residues), 37.3 bits, see alignment E=9.8e-13 PF13291: ACT_4" amino acids 628 to 704 (77 residues), 55.9 bits, see alignment E=1.7e-18

Best Hits

Swiss-Prot: 53% identical to SPOT_VIBCH: Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (spoT) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K01139, guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase [EC: 3.1.7.2] (inferred from 100% identity to psb:Psyr_0209)

Predicted SEED Role

"GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase II / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2)" in subsystem Stringent Response, (p)ppGpp metabolism (EC 2.7.6.5, EC 3.1.7.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.6.5

Use Curated BLAST to search for 2.7.6.5 or 3.1.7.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZZZ0 at UniProt or InterPro

Protein Sequence (707 amino acids)

>Psyr_0209 RelA/SpoT protein (Pseudomonas syringae pv. syringae B728a)
MARGSALPSIDALADRLSAYLGKDQVNLVRRAYFYAEQAHDGQRRRSGEAYVTHPLAVAN
ILADMHMDHQSLMAAMLHDVIEDTGIAKEALSAQFGETVAELVDGVSKLTQMNFETKAEA
QAENFQKMAMAMARDIRVILVKLADRLHNMRTLEVLSGEKRRRIAKETLEIYAPIANRLG
MHSVRIEFEDLGFKAMYPMRSSRINQAVKRARGNRKELVNKIEESLSHCLAVDGIEGDVS
GRQKHLYGIYKKMRGKRRAFNEIMDVYAFRIIVDKVDTCYRVLGAVHNLYKPLPGRFKDY
IAIPKANGYQSLHTTLFGMHGVPIEIQIRTREMEEMANNGIAAHWLYKSSDDEQPKGTHA
RARQWVKGVLEMQQRAGNSLEFIESVKIDLFPDEVYVFTPKGRIMELPKGSTAVDFAYAV
HTDVGNSCIACRINRRLAPLSEPLQSGSTVEIVSAPGARPNPAWLNFVVTGKARTHIRHA
LKLQRRSESINLGERLLNKVLNGFECSLDKIGQERIQLVLNEYRVEVLEDLLEDIGLGNR
MAYVVARRLLGEGDQLPSPEGPLAIRGTEGLVLSYAKCCTPIPGDPIVGHLSAGKGMVVH
LDNCRNISEIRHNPEKCIQLSWAKDVTGEFNVELRVELEHQRGLIALLASSVNAADGNIE
KISMDERDGRISVVQLVVSVHDRVHLARVIKKLRALTGVTRITRMRA