Protein Info for Psyr_0201 in Pseudomonas syringae pv. syringae B728a

Annotation: ATP-dependent DNA helicase RecG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 691 PF17191: RecG_wedge" amino acids 12 to 170 (159 residues), 61 bits, see alignment E=3.2e-20 TIGR00643: ATP-dependent DNA helicase RecG" amino acids 27 to 663 (637 residues), 747.4 bits, see alignment E=7.9e-229 PF04851: ResIII" amino acids 292 to 429 (138 residues), 36.3 bits, see alignment E=1.6e-12 PF00270: DEAD" amino acids 293 to 434 (142 residues), 86.2 bits, see alignment E=6.5e-28 PF00176: SNF2-rel_dom" amino acids 296 to 434 (139 residues), 25.5 bits, see alignment E=2e-09 PF00271: Helicase_C" amino acids 479 to 585 (107 residues), 64 bits, see alignment E=4.5e-21 PF19833: RecG_dom3_C" amino acids 614 to 675 (62 residues), 54.6 bits, see alignment 3e-18

Best Hits

Swiss-Prot: 58% identical to RECG_ECO57: ATP-dependent DNA helicase RecG (recG) from Escherichia coli O157:H7

KEGG orthology group: K03655, ATP-dependent DNA helicase RecG [EC: 3.6.4.12] (inferred from 100% identity to psb:Psyr_0201)

Predicted SEED Role

"ATP-dependent DNA helicase RecG (EC 3.6.1.-)" in subsystem DNA-replication (EC 3.6.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.-, 3.6.4.12

Use Curated BLAST to search for 3.6.1.- or 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZZZ8 at UniProt or InterPro

Protein Sequence (691 amino acids)

>Psyr_0201 ATP-dependent DNA helicase RecG (Pseudomonas syringae pv. syringae B728a)
MSELSRVSVTALKGVGEAMAEKLAKVGLETLQDVLFHLPLRYQDRTRIVPIGALRPGQDA
VIEGVVSGADVVMGKRRSLLVRLGDGTGVVSLRFYHFSNAQKESMKRGTHLRCFGEARPG
ASGLEIYHPEYRAITGDEPAPVEQTLTPIYPTTEGLTQQRLRQLCQQSLAMLGPKSLPDW
LPEELARDYQLAPLDDAIRYLHHPPADADVEELALGHHWAQHRLAFEELLTHQLSQQRLR
ESLRSQRAPALPVAKKLPKQFLANLGFAPTGAQQRVGKEVAYDLSQPEPMLRLIQGDVGS
GKTVVAALAALQALEAGYQVALMAPTEILAEQHYINFQRWLEPLGVGVAWLAGKLKGKAR
VASLEQIAGGTPMVVGTHALFQDEVQFKNLALVIIDEQHRFGVQQRLALRKKGVGGLMCP
HQLIMTATPIPRTLAMSAYADLDTSILDELPPGRTPVNTVLVADSRRLEVVERVRAACAE
GRQAYWVCTLIEESEELTCKAAETTYEELSSALGDVRVGLIHGRMKPAEKAAIMAEFKQG
ALQLLVATTVIEVGVDVPNSSLMIIENPERLGLAQLHQLRGRVGRGSAVSHCVLLYHPPL
SQIGRQRLGIMRETNDGFVIAEKDLELRGPGEMLGTRQTGLLQFKVADLMRDADLLPAVR
DAAQALLERWPQHVSPLLDRWLRHGQQYGQV