Protein Info for Psyr_0198 in Pseudomonas syringae pv. syringae B728a

Annotation: Citrate transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 435 signal peptide" amino acids 1 to 15 (15 residues), see Phobius details transmembrane" amino acids 22 to 44 (23 residues), see Phobius details amino acids 56 to 79 (24 residues), see Phobius details amino acids 99 to 126 (28 residues), see Phobius details amino acids 138 to 157 (20 residues), see Phobius details amino acids 174 to 195 (22 residues), see Phobius details amino acids 232 to 250 (19 residues), see Phobius details amino acids 253 to 271 (19 residues), see Phobius details amino acids 282 to 304 (23 residues), see Phobius details amino acids 316 to 342 (27 residues), see Phobius details amino acids 347 to 365 (19 residues), see Phobius details amino acids 380 to 399 (20 residues), see Phobius details amino acids 411 to 432 (22 residues), see Phobius details TIGR00784: citrate transporter" amino acids 1 to 430 (430 residues), 630.1 bits, see alignment E=1.2e-193 PF03600: CitMHS" amino acids 14 to 377 (364 residues), 134.3 bits, see alignment E=2.9e-43

Best Hits

Swiss-Prot: 62% identical to CITN_BACSU: Citrate transporter (citN) from Bacillus subtilis (strain 168)

KEGG orthology group: K03300, citrate-Mg2+:H+ or citrate-Ca2+:H+ symporter, CitMHS family (inferred from 100% identity to psb:Psyr_0198)

Predicted SEED Role

"Uncharacterized transporter, similarity to citrate transporter"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q500A1 at UniProt or InterPro

Protein Sequence (435 amino acids)

>Psyr_0198 Citrate transporter (Pseudomonas syringae pv. syringae B728a)
MLTFLGFAMVIAFMYLIMSKRLTALIALILVPIIFALFGGFASQIGPMMLAGITKLAPTG
VMLMFAILYFALMIDSGLFDPAVRKILKMVKGDPMRISVGTAVLALVVSLDGDGATTYMI
CVAAMLPLYSRIGMSPRIMAGLIILAGGVMNMTPWGGPTARAASALHVDASDIFVPMIPA
MLAGVIAILAIAYFYGKRERARLGELHLQGDEVDHSEISVSQFPDARRPKLIWFNAALTL
TLMVALIMGLLPLPVLFMIAFSIAMIINYPNLQDQKDRVSAHAGSVLAVVGLIFAAGIFT
GILSGTGMVDAMSKSLLAVIPDALGPYLAVITALVSMPFTFFMSNDAFYYGVLPVLSEAA
AHYGISPVEMARASIVGQPVHLLSPLVPSTYLLVALAGIEFGDHQRFTLKWAILVCMCIL
VAALLMGIFPFYSSM