Protein Info for Psyr_0088 in Pseudomonas syringae pv. syringae B728a

Annotation: PAS:GGDEF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 335 TIGR00229: PAS domain S-box protein" amino acids 42 to 170 (129 residues), 52.9 bits, see alignment E=3.9e-18 PF08447: PAS_3" amino acids 69 to 156 (88 residues), 71.4 bits, see alignment E=6.2e-24 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 170 to 333 (164 residues), 151.1 bits, see alignment E=2.3e-48 PF00990: GGDEF" amino acids 174 to 331 (158 residues), 142.8 bits, see alignment E=8.5e-46

Best Hits

KEGG orthology group: None (inferred from 100% identity to psb:Psyr_0088)

Predicted SEED Role

"Sensory box/GGDEF family protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q500L0 at UniProt or InterPro

Protein Sequence (335 amino acids)

>Psyr_0088 PAS:GGDEF (Pseudomonas syringae pv. syringae B728a)
MVLMEKQPPTSLPGTPITDMPAAAETLLALMHAQAEVARLSEREQLFSSLLVSVNAVLWA
FDWETQHMIYVSPAYERIFGRSAGLLLADYSEWRDSIYPDDLNYAERSLNEVIEKGSIED
REYRIIRADGEVRWLSDKCFVSHRADASQRLIVVGIAEDITEKKQLENELQRLATTDVLT
QSSNRRHFFECAQREFELARLNGTPLAFLLLDIDDFKLVNDTYGHQEGDTVLQRIAESGR
SALRRGDLFGRIGGEEFAAVFPGCAPDMAMQIAERLQREIQRQNFQSAGTTFSITASQGL
TNLGPDDPSLETLYGRADAAMYQAKRQGKNQIVLV