Protein Info for Psyr_0045 in Pseudomonas syringae pv. syringae B728a

Annotation: oligopeptidase A, Metallo peptidase, MEROPS family M03A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 683 PF19310: TOP_N" amino acids 29 to 151 (123 residues), 87 bits, see alignment E=9e-29 PF01432: Peptidase_M3" amino acids 225 to 676 (452 residues), 510.3 bits, see alignment E=7.4e-157

Best Hits

Swiss-Prot: 60% identical to OPDA_SALTY: Oligopeptidase A (prlC) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K01414, oligopeptidase A [EC: 3.4.24.70] (inferred from 100% identity to psb:Psyr_0045)

MetaCyc: 60% identical to oligopeptidase A (Escherichia coli K-12 substr. MG1655)
Oligopeptidase A. [EC: 3.4.24.70]

Predicted SEED Role

"Oligopeptidase A (EC 3.4.24.70)" in subsystem Protein degradation (EC 3.4.24.70)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.4.24.70

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q500Q3 at UniProt or InterPro

Protein Sequence (683 amino acids)

>Psyr_0045 oligopeptidase A, Metallo peptidase, MEROPS family M03A (Pseudomonas syringae pv. syringae B728a)
MSANNPLLQSYDLPPFSAIRAEHVKPAIEQILADNRAAIADILAKQGSTPTWAGLVLTMD
ELNDRLGAAWSPVSHLNAVCNSAELREAYESCLPALSAYSTEMGQNRALFQAYEALANGP
EAASFDVGQKTILEQSLRDFRLSGIDLPPEQQKRYAEVQSKLSELGSQFSNQLLDATQAW
TKLVADESALAGLTDSAKQQMAAAAKAKDLEGYLITLEFPNYYAVMTYAEDRALREEVYA
AYATRASDQGPNAGKNDNTPVMEQILDLRQELAQLLGYANYAELSLATKMAESSDQVLSF
LRDLAKRSKPFAAQDLEQLKAYAAEQGCPDLQSWDSGFYGEKLREQRYSVSQEILRAYFP
VDKVLDGLFTIVQRLYGIEIAEQKGFDTWHPDVRLFEIKENGQHVGRFFFDLYARANKRG
GAWMDGARDRRRTAQGTLQSPVANLVCNFTPAVAGKPALLTHDEVTTLFHEFGHGLHHLL
TRVEHAGVSGINGVAWDAVELPSQFMENWCWEPEGLALISGHYETGEPLPQDLLEKMLAA
KNFQSGLMMVRQLEFSLFDFELHATHGDGRSVLEVLEGIRDEVSVMRPPAYNRFPNSFAH
IFAGGYAAGYYSYKWAEVLSADAFSKFEEDGVLNAETGRAFREAILARGGSQAPMVLFVD
FRGREPSIDALLRHSGLSEDAAA