Protein Info for Psyr_0011 in Pseudomonas syringae pv. syringae B728a

Annotation: glycyl-tRNA synthetase beta chain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 684 TIGR00211: glycine--tRNA ligase, beta subunit" amino acids 1 to 683 (683 residues), 800.3 bits, see alignment E=1.1e-244 PF02092: tRNA_synt_2f" amino acids 6 to 544 (539 residues), 683.3 bits, see alignment E=3.1e-209 PF05746: DALR_1" amino acids 579 to 671 (93 residues), 36.1 bits, see alignment E=6.1e-13

Best Hits

Swiss-Prot: 100% identical to SYGB_PSEU2: Glycine--tRNA ligase beta subunit (glyS) from Pseudomonas syringae pv. syringae (strain B728a)

KEGG orthology group: K01879, glycyl-tRNA synthetase beta chain [EC: 6.1.1.14] (inferred from 100% identity to psb:Psyr_0011)

Predicted SEED Role

"Glycyl-tRNA synthetase beta chain (EC 6.1.1.14)" (EC 6.1.1.14)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.14

Use Curated BLAST to search for 6.1.1.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q500T7 at UniProt or InterPro

Protein Sequence (684 amino acids)

>Psyr_0011 glycyl-tRNA synthetase beta chain (Pseudomonas syringae pv. syringae B728a)
MSAQDFLVELGTEELPPKTLVSLADAFLAGIEKGLAGAGLTYSAKQVYAAPRRLAVLITA
LATQQPDRSVNLDGPPRQAAFDADGNPTQAALGFAKKCGVDLSEIDQSGPKLRYSQTILG
KPTTSLLPTIVEDSLNDLPIAKRMRWGARKEEFVRPTQWLVMLFGDQVVDCTILAQSAGR
HSRGHRFHHPQDVRISSPAGYLSDLRAAHVLADFNERRQIISKRVDELATQQEGTAIVPP
SLLDEVAGLVEWPVPLVCSFEERFLEVPQEALITTMQDNQKYFCLLDVDGKLLPRFITVA
NIESKDPAQIIAGNEKVVRPRLTDAEFFFKQDKKQKLETFNDRLKNVVFQAQLGSVFDKA
ERVSKLAAYIAPRIGGDAQRAARAGLLSKCDLSSEMVGEFPEMQGIAGYYYAKADGEPED
VALALNEQYMPRGAGAELPTTLTGAAVAIADKLDTLVGIFGIGMLPTGSKDPYALRRAAL
GILRILIEKKLDLNLVETVKFAVAQFGAKIKPAGLAEQVLDFIFDRLRARYEDEGVDVAV
YLSVRALQPASALDFDQRVQAVQAFRKLPQAAALAAVNKRVSNLLSKAEGSIAQTVEPKY
FDNANEFSLYSAIQQADHAIQPMAAERQYSESLARLAMLREPVDAFFEAVMVNAEDANVR
ANRYALLSRLRGLFLGVADISLLG